Vertical Distribution of Bacterial Community Diversity and Water Quality during the Reservoir Thermal Stratification

Hai-Han Zhang, Sheng-Nan Chen, Ting-Lin Huang, Wei-Xing Ma, Jin-Lan Xu and Xin Sun
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Hai-Han Zhang: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China
Sheng-Nan Chen: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China
Ting-Lin Huang: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China
Wei-Xing Ma: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China
Jin-Lan Xu: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China
Xin Sun: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No.13, YanTa Road, Xi'an 710055, China

IJERPH, 2015, vol. 12, issue 6, 1-13

Abstract: Reservoir thermal stratification drives the water temperature and dissolved oxygen gradient, however, the characteristic of vertical water microbial community during thermal stratification is so far poorly understood. In this work, water bacterial community diversity was determined using the Illumina Miseq sequencing technique. The results showed that epilimnion, metalimnion and hypolimnion were formed steadily in the JINPEN drinking water reservoir. Water temperature decreased steadily from the surface (23.11 °C) to the bottom (9.17 °C). Total nitrogen ranged from 1.07 to 2.06 mg/L and nitrate nitrogen ranged from 0.8 to 1.84 mg/L. The dissolved oxygen concentration decreased sharply below 50 m, and reached zero at 65 m. The Miseq sequencing revealed a total of 4127 operational taxonomic units (OTUs) with 97% similarity, which were affiliated with 15 phyla including Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, Caldiserica, Chlamydiae, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The highest Shannon diversity was 4.41 in 45 m, and the highest Chao 1 diversity was 506 in 5 m. Rhodobacter dominated in 55 m (23.24%) and 65 m (12.58%). Prosthecobacter dominated from 0.5 to 50 m. The heat map profile and redundancy analysis (RDA) indicated significant difference in vertical water bacterial community composition in the reservoir. Meanwhile, water quality properties including dissolved oxygen, conductivity, nitrate nitrogen and total nitrogen have a dramatic influence on vertical distribution of bacterial communities.

Keywords: reservoir; thermal stratification; water bacterial community; high-throughput sequencing (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
References: View complete reference list from CitEc
Citations: View citations in EconPapers (6)

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