Extraction of 3,4,4?-Trichlorocarbanilide from Rat Fecal Samples for Determination by High Pressure Liquid Chromatography–Tandem Mass Spectrometry

Rebekah C. Kennedy, Russell R. Fling, Paul D. Terry, Fu-Min Menn, Jiangang Chen and Christopher J. Borman
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Rebekah C. Kennedy: Comparative and Experimental Medicine, University of Tennessee, Knoxville, TN 37996, USA
Russell R. Fling: Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA
Paul D. Terry: Department of Public Health, University of Tennessee, Knoxville, TN 37996, USA
Fu-Min Menn: Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA
Jiangang Chen: Department of Public Health, University of Tennessee, Knoxville, TN 37996, USA
Christopher J. Borman: Department of Natural Sciences, Heritage University, Toppenish, WA 98948, USA

IJERPH, 2015, vol. 12, issue 7, 1-8

Abstract: Triclocarban (3,4,4?-Trichlorocarbanilide; TCC) in the environment has been well documented. Methods have been developed to monitor TCC levels from various matrices including water, sediment, biosolids, plants, blood and urine; however, no method has been developed to document the concentration of TCC in fecal content after oral exposure in animal studies. In the present study, we developed and validated a method that uses liquid extraction coupled with HPLC-MS/MS determination to measure TCC in feces. The limit of detection and limit of quantitation in control rats without TCC exposure was 69.0 ng/g and 92.9 ng/g of feces, respectively. The base levels of TCC in feces were lower than LOD. At 12 days of treatment, the fecal TCC concentration increased to 2220 µg/g among 0.2% w/w exposed animals. The concentration in fecal samples decreased over the washout period in 0.2% w/w treated animals to 0.399 µ/g feces after exposure was removed for 28 days. This method required a small amount of sample (0.1 g) with simple sample preparation. Given its sensitivity and efficiency, this method may be useful for monitoring TCC exposure in toxicological studies of animals.

Keywords: triclocarban; antimicrobials; feces; HPLC-MS/MS (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2015
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