Overexpression of RACK1 Promotes Metastasis by Enhancing Epithelial-Mesenchymal Transition and Predicts Poor Prognosis in Human Glioma

Qiao-Li Lv, Yuan-Tao Huang, Gui-Hua Wang, Yan-Ling Liu, Jin Huang, Qiang Qu, Bao Sun, Lei Hu, Lin Cheng, Shu-Hui Chen and Hong-Hao Zhou
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Qiao-Li Lv: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Yuan-Tao Huang: Department of Neurology, The Brain Hospital of Hunan Province, Changsha 410008, China
Gui-Hua Wang: Department of Oncology, Changsha Central Hospital, Changsha 410008, China
Yan-Ling Liu: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Jin Huang: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Qiang Qu: Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China
Bao Sun: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Lei Hu: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China
Lin Cheng: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510275, China
Shu-Hui Chen: Department of Oncology, Changsha Central Hospital, Changsha 410008, China
Hong-Hao Zhou: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China

IJERPH, 2016, vol. 13, issue 10, 1-15

Abstract: Emerging studies show that dysregulation of the receptor of activated protein kinase C1 (RACK1) plays a crucial role in tumorigenesis and progression of various cancers. However, the biological function and underlying mechanism of RACK1 in glioma remains poorly defined. Here, we found that RACK1 was significantly up-regulated in glioma tissues compared with normal brain tissues, being closely related to clinical stage of glioma both in mRNA and protein levels. Moreover, Kaplan-Meier analysis demonstrated that patients with high RACK1 expression had a poor prognosis ( p = 0.0062, HR = 1.898, 95% CI: 1.225–3.203). In vitro functional assays indicated that silencing of RACK1 could dramatically promote apoptosis and inhibit cell proliferation, migration, and invasion of glioma cells. More importantly, knockdown of RACK1 led to a vast accumulation of cells in G0/G1 phase and their reduced proportions at the S phase by suppressing the expression of G1/S transition key regulators Cyclin D1 and CDK6. Additionally, this forced down-regulation of RACK1 significantly suppressed migration and invasion via inhibiting the epithelial-mesenchymal transition (EMT) markers, such as MMP2, MMP9, ZEB1, N-Cadherin, and Integrin-β1. Collectively, our study revealed that RACK1 might act as a valuable prognostic biomarker and potential therapeutic target for glioma.

Keywords: RACK1; cell cycle; epithelial-mesenchymal transition; prognosis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
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