Changes in Composition and Function of Human Intestinal Microbiota Exposed to Chlorpyrifos in Oil as Assessed by the SHIME ® Model

Julie Reygner, Claire Joly Condette, Aurélia Bruneau, Stéphane Delanaud, Larbi Rhazi, Flore Depeint, Latifa Abdennebi-Najar, Veronique Bach, Camille Mayeur and Hafida Khorsi-Cauet
Additional contact information
Julie Reygner: Laboratoire Périnatalité et Risques Toxiques (PERITOX), UMR-I01 INERIS, UPJV-CURS CHU Sud, Avenue Laennec, F-80054 Amiens CEDEX 1, France
Claire Joly Condette: Laboratoire Périnatalité et Risques Toxiques (PERITOX), UMR-I01 INERIS, UPJV-CURS CHU Sud, Avenue Laennec, F-80054 Amiens CEDEX 1, France
Aurélia Bruneau: INRA, UMR1319 MICALIS, AMIPEM, F-78350 Jouy-en-Josas, France
Stéphane Delanaud: Laboratoire Périnatalité et Risques Toxiques (PERITOX), UMR-I01 INERIS, UPJV-CURS CHU Sud, Avenue Laennec, F-80054 Amiens CEDEX 1, France
Larbi Rhazi: UP-EGEAL 2012.10.101, Institut Polytechnique LaSalle Beauvais, 19 Rue Pierre Waguet, F-60026 Beauvais CEDEX, France
Flore Depeint: UP-EGEAL 2012.10.101, Institut Polytechnique LaSalle Beauvais, 19 Rue Pierre Waguet, F-60026 Beauvais CEDEX, France
Latifa Abdennebi-Najar: UP-EGEAL 2012.10.101, Institut Polytechnique LaSalle Beauvais, 19 Rue Pierre Waguet, F-60026 Beauvais CEDEX, France
Veronique Bach: Laboratoire Périnatalité et Risques Toxiques (PERITOX), UMR-I01 INERIS, UPJV-CURS CHU Sud, Avenue Laennec, F-80054 Amiens CEDEX 1, France
Camille Mayeur: AgroParisTech, UMR1319 MICALIS, F-78350 Jouy-en-Josas, France
Hafida Khorsi-Cauet: Laboratoire Périnatalité et Risques Toxiques (PERITOX), UMR-I01 INERIS, UPJV-CURS CHU Sud, Avenue Laennec, F-80054 Amiens CEDEX 1, France

IJERPH, 2016, vol. 13, issue 11, 1-18

Abstract: The presence of pesticide residues in food is a public health problem. Exposure to these substances in daily life could have serious effects on the intestine—the first organ to come into contact with food contaminants. The present study investigated the impact of a low dose (1 mg/day in oil) of the pesticide chlorpyrifos (CPF) on the community structure, diversity and metabolic response of the human gut microbiota using the SHIME ® model (six reactors, representing the different parts of the gastrointestinal tract). The last three reactors (representing the colon) were inoculated with a mixture of feces from human adults. Three time points were studied: immediately before the first dose of CPF, and then after 15 and 30 days of CPF-oil administration. By using conventional bacterial culture and molecular biology methods, we showed that CPF in oil can affect the gut microbiota. It had the greatest effects on counts of culturable bacteria (with an increase in Enterobacteria, Bacteroides spp. and clostridia counts, and a decrease in bifidobacterial counts) and fermentative activity, which were colon-segment-dependent. Our results suggest that: (i) CPF in oil treatment affects the gut microbiota (although there was some discordance between the culture-dependent and culture-independent analyses); (ii) the changes are “SHIME ® -compartment” specific; and (iii) the changes are associated with minor alterations in the production of short-chain fatty acids and lactate.

Keywords: pesticide; microbiota; human fecal ecosystem; diversity; functional activity; SHIME ® model (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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