Simultaneous Detection of Five Pathogens from Cerebrospinal Fluid Specimens Using Luminex Technology

Linfu Zhou, Rui Wu, Xiaodan Shi, Dongyun Feng, Guodong Feng, Yining Yang, Wen Dai, Ting Bian, Tingting Liu, Ying He, Ming Shi and Gang Zhao
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Linfu Zhou: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Rui Wu: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Xiaodan Shi: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Dongyun Feng: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Guodong Feng: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Yining Yang: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Wen Dai: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Ting Bian: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Tingting Liu: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Ying He: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Ming Shi: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
Gang Zhao: Department of Neurology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China

IJERPH, 2016, vol. 13, issue 2, 1-11

Abstract: Early diagnosis and treatment are crucial for the outcome of central nervous system (CNS) infections. In this study, we developed a multiplex PCR-Luminex assay for the simultaneous detection of five major pathogens, including Mycobacterium tuberculosis , Cryptococcus neoformans , Streptococcus pneumoniae , and herpes simplex virus types 1 and 2, which frequently cause CNS infections. Through the hybridization reaction between multiplex PCR-amplified targets and oligonucleotide “anti-TAG” sequences, we found that the PCR-Luminex assay could detect as low as 10 1 –10 2 copies of synthetic pathogen DNAs. Furthermore, 163 cerebrospinal fluid (CSF) specimens from patients with suspected CNS infections were used to evaluate the efficiency of this multiplex PCR-Luminex method. Compared with Ziehl-Neelsen stain, this assay showed a high diagnostic accuracy for tuberculosis meningitis (sensitivity, 90.7% and specificity, 99.1%). For cryptococcal meningitis, the sensitivity and specificity were 92% and 97.1%, respectively, compared with the May Grunwald Giemsa (MGG) stain. For herpes simplex virus types 1 and 2 encephalitis, the sensitivities were 80.8% and 100%, and the specificities were 94.2% and 99%, respectively, compared with Enzyme Linked Immunosorbent Assay (ELISA) assays. Taken together, this multiplex PCR-Luminex assay showed potential efficiency for the simultaneous detection of five pathogens and may be a promising supplement to conventional methods for diagnosing CNS infections.

Keywords: Luminex technology; multiplex PCR; central nervous system infections; cerebrospinal fluid (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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