Protective Effect of Onion Extract on Bleomycin-Induced Cytotoxicity and Genotoxicity in Human Lymphocytes

Yoon Hee Cho, Joong Won Lee, Hae Dong Woo, Sunyeong Lee, Yang Jee Kim, Younghyun Lee, Sangah Shin, Hyojee Joung and Hai Won Chung
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Yoon Hee Cho: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Joong Won Lee: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Hae Dong Woo: Molecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Ilsandong-gu, Gyeonggi-do 410-769, Korea
Sunyeong Lee: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Yang Jee Kim: Da Vinci College of General Education, Chung-Ang University, Dongjak-Gu, Seoul 156-756, Korea
Younghyun Lee: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Sangah Shin: Institute of Health and Environment, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Hyojee Joung: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
Hai Won Chung: School of Public Health, Seoul National University, Gwanak-gu, Seoul 151-742, Korea

IJERPH, 2016, vol. 13, issue 2, 1-11

Abstract: Following one of the world’s largest nuclear accidents, occured at Fukushima, Japan in 2011, a significant scientific effort has focused on minimizing the potential adverse health effects due to radiation exposure. The use of natural dietary antioxidants to reduce the risk of radiation-induced oxidative DNA damage is a simple strategy for minimizing radiation-related cancer rates and improving overall health. The onion is among the richest sources of dietary flavonoids and is an important food for increasing their overall intake. Therefore, we examined the effect of an onion extract on cyto- and geno-toxicity in human lymphocytes treated with bleomycin (BLM), a radiomimetic agent. In addition, we measured the frequency of micronuclei (MN) and DNA damage following treatment with BLM using a cytokinesis-blocked micronucleus assay and a single cell gel electrophoresis assay. We observed a significant increase in cell viability in lymphocytes treated with onion extract then exposed to BLM compared to cells treated with BLM alone. The frequency of BLM induced MN and DNA damage increased in a dose-dependent manner; however, when lymphocytes were pretreated with onion extract (10 and 20 ?L/mL), the frequency of BLM-induced MN was decreased at all doses of BLM and DNA damage was decreased at 3 ?g/mL of BLM. These results suggest that onion extract may have protective effects against BLM-induced cyto- and genotoxicity in human lymphocytes.

Keywords: radiation; onion extract; bleomycin; cytokinesis-blocked micronucleus assay; single cell gel electrophoresis assay (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
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