Predicting the Cytotoxic Potency of Cigarette Smoke by Assessing the Thioredoxin Reductase Inhibitory Capacity of Cigarette Smoke Extract

Longjie Zhang, Min Ning, Yingbo Xu, Chenghui Wang, Guangshan Zhao, Qingqing Cao and Jinsong Zhang
Additional contact information
Longjie Zhang: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China
Min Ning: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China
Yingbo Xu: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China
Chenghui Wang: Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China
Guangshan Zhao: State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China
Qingqing Cao: State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China
Jinsong Zhang: State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China

IJERPH, 2016, vol. 13, issue 3, 1-13

Abstract: The present study investigated the influence of the cigarette smoke extract (CSE) on mammalian thioredoxin reductase (TrxR) activity. TrxR is a selenoenzyme with a selenocysteine (Sec) residue exposed on the enzyme’s surface. This unique Sec residue is particularly susceptible to modification by numerous types of electrophiles, leading to inactivation of TrxR and consequent cytotoxicity. Cigarette smoke contains various electrophiles, and the present study showed that CSE could inhibit intracellular TrxR through causing crosslinking and alkylation of TrxR1. TrxR inhibitory capacities of various CSEs were evaluated by using mouse-liver homogenate. Among the CSEs prepared from 18 commercial cigarette brands, TrxR inhibitory capacities of the maximum and the minimum had a 2.5-fold difference. Importantly, CSE’s inhibitory capacity greatly paralleled its cytotoxic potency in all cell lines used. Compared to cytotoxic assays, which have been widely used for evaluating cigarette toxicity but are not suitable for simultaneously examining a large number of cigarette samples, the present method was simple and rapid with a high-throughput feature and thus could be used as an auxiliary means to predict the cytotoxicity of a large number of cigarette samples, making it possible to extensively screen numerous agricultural and industrial measures that potentially affect cigarette safety.

Keywords: cigarette smoke; thioredoxin reductase; cytotoxicity; high throughput (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/13/3/348/pdf (application/pdf)
https://www.mdpi.com/1660-4601/13/3/348/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:13:y:2016:i:3:p:348-:d:66186

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().