Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress

Yue Wang, Sheng-Yuan Wang, Li Jia, Lin Zhang, Jing-Chong Ba, Dan Han, Cui-Ping Yu and Yong-Hui Wu
Additional contact information
Yue Wang: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Sheng-Yuan Wang: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Li Jia: Department of Environmental Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Lin Zhang: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Jing-Chong Ba: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Dan Han: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Cui-Ping Yu: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China
Yong-Hui Wu: Department of Occupational Health, College of Public Health, Harbin Medical University, Harbin 150000, China

IJERPH, 2016, vol. 13, issue 7, 1-15

Abstract: Although there have been numerous studies examining the toxicity and carcinogenicity of nickel compounds in humans and animals, its molecular mechanisms of action are not fully elucidated. In our research, NIH/3T3 cells were exposed to nickel-refining fumes at the concentrations of 0, 6.25, 12.50, 25, 50 and 100 ?g/mL for 24 h. Cell viability, cell apoptosis, reactive oxygen species (ROS) level, lactate dehydrogenase (LDH) assay, the level of glutathione (GSH), activities of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) level were detected. The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in a dose-dependent manner. Nickel-refining fumes significantly increased ROS levels and induced DNA damage. Nickel-refining fumes may induce the changes in the state of ROS, which may eventually initiate oxidative stress, DNA damage and apoptosis of NIH/3T3 cells.

Keywords: nickel-refining fumes; NIH/3T3cell; DNA damage; apoptosis; oxidative stress (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/13/7/629/pdf (application/pdf)
https://www.mdpi.com/1660-4601/13/7/629/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:13:y:2016:i:7:p:629-:d:72625

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().