Surface Water Impacted by Rural Activities Induces Genetic Toxicity Related to Recombinagenic Events in Vivo

José Lopes Soares Neto, Raíne Fogliati De Carli, Queila Susana Gambim Kotzal, Francine Bolico Latroni, Mauricio Lehmann, Johnny Ferraz Dias, Cláudia Telles De Souza, Liana Appel Boufleur Niekraszewicz, Fernanda Rabaioli Da Silva, Juliana Da Silva and Rafael Rodrigues Dihl
Additional contact information
José Lopes Soares Neto: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Raíne Fogliati De Carli: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Queila Susana Gambim Kotzal: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Francine Bolico Latroni: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Mauricio Lehmann: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Johnny Ferraz Dias: Ion Implantation Laboratory, Institute of Physics, UFRGS, Porto Alegre 91501-970, Brazil
Cláudia Telles De Souza: Ion Implantation Laboratory, Institute of Physics, UFRGS, Porto Alegre 91501-970, Brazil
Liana Appel Boufleur Niekraszewicz: Ion Implantation Laboratory, Institute of Physics, UFRGS, Porto Alegre 91501-970, Brazil
Fernanda Rabaioli Da Silva: Ion Implantation Laboratory, Institute of Physics, UFRGS, Porto Alegre 91501-970, Brazil
Juliana Da Silva: Laboratory of Genetic Toxicology, Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil
Rafael Rodrigues Dihl: Laboratory of Genetic Toxicity (TOXIGEN), Post-Graduation Program in Molecular and Cellular Biology Applied to Health, Lutheran University of Brazil (ULBRA), Canoas 92425-900, Brazil

IJERPH, 2016, vol. 13, issue 8, 1-12

Abstract: This investigation assessed the interaction of surface water samples with DNA to quantitatively and qualitatively characterize their mutagenic and/or recombinagenic activity. Samples were obtained at three different sites along the Tocantins River (Tocantins State, Brazil). The area has withstood the impact mainly of rural activities, which release different chemical compounds in the environment. The Drosophila melanogaster Somatic Mutation and Recombination Test (SMART) was performed in standard (ST) and high bioactivation (HB) crosses. SMART is useful for the detection of mutational and recombinational events induced by genotoxins of direct and indirect action. Results demonstrated that samples collected in both seasons were able to induce increments on the mutant spot frequencies in the larvae of the HB cross. Genotoxicity was related to a massive recombinagenic activity. The positive responses ascribed to only the HB cross means that it is linked to pro-genotoxins requiring metabolic activation. The SMART wing test in Drosophila melanogaster was shown to be highly sensitive to detect genotoxic agents present in the aquatic environment impacted by agriculture.

Keywords: agriculture; genotoxicity; somatic cells; surface water; SMART (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2016
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/13/8/827/pdf (application/pdf)
https://www.mdpi.com/1660-4601/13/8/827/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:13:y:2016:i:8:p:827-:d:76073

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().