Characterization of Diarrheagenic Escherichia coli Isolated in Organic Waste Products (Cattle Fecal Matter, Manure and, Slurry) from Cattle’s Markets in Ouagadougou, Burkina Faso

Evariste Bako, Assèta Kagambèga, Kuan Abdoulaye Traore, Touwendsida Serge Bagre, Hadiza Bawa Ibrahim, Soutongnooma Caroline Bouda, Isidore Juste Ouindgueta Bonkoungou, Saidou Kaboré, Cheikna Zongo, Alfred Sababenejo Traore and Nicolas Barro
Additional contact information
Evariste Bako: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Assèta Kagambèga: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Kuan Abdoulaye Traore: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Touwendsida Serge Bagre: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Hadiza Bawa Ibrahim: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Soutongnooma Caroline Bouda: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Isidore Juste Ouindgueta Bonkoungou: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Saidou Kaboré: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso
Cheikna Zongo: Laboratoire de Biochimie et d’Immunologie Appliquée (LABIA), Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Professeur Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina Faso
Alfred Sababenejo Traore: Centre de Recherche en Sciences Biologiques Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Professeur Joseph KI-ZERBO, 03 BP 7131 Ouagadougou 03, Burkina Faso
Nicolas Barro: Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga I Prof Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

IJERPH, 2017, vol. 14, issue 10, 1-12

Abstract: Cattle farming can promote diarrheal disease transmission through waste, effluents or cattle fecal matter. The study aims to characterize the diarrheagenic Escherichia coli (DEC) isolated from cattle feces, manure in the composting process and slurry, collected from four cattle markets in Ouagadougou. A total of 585 samples (340 cattle feces, 200 slurries and 45 manures in the composting process) were collected from the four cattle markets between May 2015 and May 2016. A multiplex Polymerase Chain Reaction (PCR), namely 16-plex PCR, was used to screen simultaneously the virulence genes specific for shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC) and enteroaggregative E. coli (EAEC). DEC was detected in 10.76% of samples. ETEC was the most prevalent (9.91%). STEC and EAEC have been observed with the same rate (0.51%). ETEC were detected in 12.64% of cattle feces, in 6.66% of manure in the composting process and in 5% of slurry. STEC were detected in 0.58% of cattle feces and in 2.22% of manure in the composting process. EAEC was detected only in 1% of slurry and in 2.22% of manure in the composting process. ETEC strains were identified based on estIa gene and/or estIb gene and/or elt gene amplification. Of the 58 ETEC, 10.34% contained astA , 17.24% contained elt , 3.44% contained estIa and 79.31% contained estIb . The two positive EAEC strains contained only the aggR gene, and the third was positive only for the pic gene. The results show that effluent from cattle markets could contribute to the spreading of DEC in the environment in Burkina Faso.

Keywords: manure; cattle fecal matter; STEC; ETEC; EAEC; slurries; cattle market; diarrheal diseases; environment sanitation; public health (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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