Antibiotic Susceptibility, Genetic Diversity, and the Presence of Toxin Producing Genes in Campylobacter Isolates from Poultry

Jeeyeon Lee, Jiyeon Jeong, Heeyoung Lee, Jimyeong Ha, Sejeong Kim, Yukyung Choi, Hyemin Oh, Kunho Seo, Yohan Yoon and Soomin Lee
Additional contact information
Jeeyeon Lee: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Jiyeon Jeong: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Heeyoung Lee: Risk Analysis Research Center, Sookmyung Women’s University, Seoul 04310, Korea
Jimyeong Ha: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Sejeong Kim: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Yukyung Choi: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Hyemin Oh: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Kunho Seo: Department of Veterinary Medicine, Konkuk University, Seoul 05029, Korea
Yohan Yoon: Department of Food and Nutrition, Sookmyung Women’s University, Seoul 04310, Korea
Soomin Lee: Risk Analysis Research Center, Sookmyung Women’s University, Seoul 04310, Korea

IJERPH, 2017, vol. 14, issue 11, 1-11

Abstract: This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in Campylobacter isolates from poultry. Chicken ( n = 152) and duck ( n = 154) samples were collected from 18 wet markets in Korea. Campylobacter spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLab TM system and were tested for the presence of cytolethal distending toxin ( cdt ) genes. Campylobacter spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of Campylobacter contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three cdt genes ( cdtA , cdtB , and cdtC ) were present in 71.1% of Campylobacter isolates. These results indicate that Campylobacter contamination should be decreased to prevent and treat Campylobacter foodborne illness.

Keywords: Campylobacter; poultry; antibiotic susceptibility; Rep-PCR; cdt toxin (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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