Optimization of the Use of His 6 -OPH-Based Enzymatic Biocatalysts for the Destruction of Chlorpyrifos in Soil

Olga Senko, Olga Maslova and Elena Efremenko
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Olga Senko: Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
Olga Maslova: Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
Elena Efremenko: Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia

IJERPH, 2017, vol. 14, issue 12, 1-15

Abstract: Applying enzymatic biocatalysts based on hexahistidine-containing organophosphorus hydrolase (His 6 -OPH) is suggested for the decomposition of chlorpyrifos, which is actively used in agriculture in many countries. The application conditions were optimized and the following techniques was suggested to ensure the highest efficiency of the enzyme: first, the soil is alkalinized with hydrated calcitic lime Ca(OH) 2 , then the enzyme is introduced into the soil at a concentration of 1000 U/kg soil. Non-equilibrium low temperature plasma (NELTP)-modified zeolite is used for immobilization of the relatively inexpensive polyelectrolyte complexes containing the enzyme His 6 -OPH and a polyanionic polymer: poly- l -glutamic acid (PLE 50 ) or poly- l -aspartic acid (PLD 50 ). The soil’s humidity is then increased up to 60–80%, the top layer (10–30 cm) of soil is thoroughly stirred, and then exposed for 48–72 h. The suggested approach ensures 100% destruction of the pesticide within 72 h in soils containing as much as 100 mg/kg of chlorpyrifos. It was concluded that using this type of His 6 -OPH-based enzyme chemical can be the best approach for soils with relatively low humus concentrations, such as sandy and loam-sandy chestnut soils, as well as types of soil with increased alkalinity (pH 8.0–8.4). Such soils are often encountered in desert, desert-steppe, foothills, and subtropical regions where chlorpyrifos is actively used.

Keywords: hexahistidine-containing organophosphorus hydrolase; soil; chlorpyrifos biodegradation; Dursban; agricultural pesticides; organophosphorus and organochlorine compounds (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
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