Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Tomislav Kizivat, Martina Smolić, Ivana Marić, Maja Tolušić Levak, Robert Smolić, Ines Bilić Čurčić, Lucija Kuna, Ivan Mihaljević, Aleksandar Včev and Sandra Tucak-Zorić
Additional contact information
Tomislav Kizivat: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Martina Smolić: Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10, HR-3100 Osijek, Croatia
Ivana Marić: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Maja Tolušić Levak: Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10, HR-3100 Osijek, Croatia
Robert Smolić: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Ines Bilić Čurčić: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Lucija Kuna: Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10, HR-3100 Osijek, Croatia
Ivan Mihaljević: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Aleksandar Včev: Clinical Hospital Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia
Sandra Tucak-Zorić: Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10, HR-3100 Osijek, Croatia

IJERPH, 2017, vol. 14, issue 1, 1-13

Abstract: Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of antioxidant pre-treatment on inhibition of oxidative stress. Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l -arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). In all three cell lines, pre-treatment of antioxidants increased cell survival. Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. Our results indicate that an antioxidant pre-treatment with l -arginine and vitamin E is able to hamper oxalate-induced oxidative stress in kidney epithelial cells and as such could play a role in prevention of urolithiasis.

Keywords: urolithiasis; vitamin E; l -arginine; Madin-Darby canine kidney cells; LLC-PK1 cells (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/14/1/109/pdf (application/pdf)
https://www.mdpi.com/1660-4601/14/1/109/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:14:y:2017:i:1:p:109-:d:88593

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().