Effects of Ambient Fine Particles PM 2.5 on Human HaCaT Cells

Qiao Li, Zhihua Kang, Shuo Jiang, Jinzhuo Zhao, Shuxian Yan, Feng Xu and Jinhua Xu
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Qiao Li: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Zhihua Kang: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Shuo Jiang: Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China
Jinzhuo Zhao: Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China
Shuxian Yan: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Feng Xu: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
Jinhua Xu: Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China

IJERPH, 2017, vol. 14, issue 1, 1-10

Abstract: The current study was conducted to observe the effects of fine particulate matter (PM 2.5 ) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM 2.5 and skin was explored. HaCaT cells were cultured and then accessed in plate with PM 2.5 . Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-?, Interleukin-1?, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM 2.5 . Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM 2.5 treatment groups. The level of Tumor Necrosis Factor-?, Thymic Stromal Lymphopoietin, Interleukin-1?, and Interleukin-8 significantly increased in the cells treated with PM 2.5 . Ambient PM 2.5 may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.

Keywords: PM 2.5; HaCaT cell; skin damage (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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