Possible Impact of 190G > A CCR2 and ?32 CCR5 Mutations on Decrease of the HBV Vaccine Immunogenicity—A Preliminary Report

Maria Ganczak, Karolina Skonieczna-Żydecka, Marzena Drozd-Dąbrowska and Grażyna Adler
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Maria Ganczak: Department of Epidemiology and Management, Pomeranian Medical University, Szczecin 70-204, Poland
Karolina Skonieczna-Żydecka: Department of Gerontobiology, Pomeranian Medical University, Szczecin 70-204, Poland
Marzena Drozd-Dąbrowska: Department of Epidemiology and Management, Pomeranian Medical University, Szczecin 70-204, Poland
Grażyna Adler: Department of Gerontobiology, Pomeranian Medical University, Szczecin 70-204, Poland

IJERPH, 2017, vol. 14, issue 2, 1-9

Abstract: Background : Chemokine genetic variations are involved in infectious diseases such as hepatitis B virus (HBV). Several allelic variants might, in theory, affect the outcome of vaccination. Objectives : This study was carried out to examine the associations of ?32 CCR5 and 190G > A CCR2 polymorphisms with a response to a primary course of three HBV vaccinations. Methods : Between December 2014 and December 2016, patients from three randomly selected primary care clinics in the West Pomeranian region (Poland), 1 month after receiving the third dose of HBV vaccine, were enrolled. Enzyme-linked immunosorbent assay (ELISA) system version 3.0 was used to detect anti-HBs and anti-HBc totals. The identification of polymorphisms were performed by a polymerase chain reaction technique using a single primer extension assay. Genotype distributions of responders versus non-responders to HBV vaccination were compared on the basis of anti-HBs level. Results : In 149 patients (mean age 60 years) the mean anti-HBs level was 652.2 ± 425.9 mIU/mL (range: 0–1111.0 mIU/mL). There were 14.1% ( n = 21) non-responders to the HBV vaccine (anti-HBs < 10.0 mIU/mL). The wild type/?32 genotype of CCR5 gene was found in 18.1% participants, and 1.3% were ?32/?32 homozygotes. The frequency of allele A of the CCR2 gene was 11.1%. Lower anti-HBs levels in ?32/?32 homozygotes were observed (Me = 61 mIU/mL vs. Me = 660.2 mIU/mL; p = 0.048). As age was found to be a correlate to the anti-HBs titer ( r = ?0.218, p = 0.0075; 95% CI: ?0.366–?0.059)—an analysis of a co-variance was performed which found a statistically significant ( p = 0.04) difference in anti-HBs titres between ?32/?32 homozygotes and other CCR5 genotypes. The association between anti-HBs titres and CCR2 genotypes was not statistically significant. Conclusions : Our study—which is a preliminary report that suggest this topic deserves further observation with larger sample sizes, different ethnicities, and other single nucleotide poly-morphisms (SNPs)—suggests the possible involvement of CCR5 polymorphism in impairing the immunologic response to HBV vaccination, predominantly in relation to the passage of time.

Keywords: HBV; vaccination; immunogenicity; CCR5; CCR2; polymorphism (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
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