DNA Methylation Status of PAX1 and ZNF582 in Esophageal Squamous Cell Carcinoma

Jin Huang, Guo Wang, Jie Tang, Wei Zhuang, Li-Ping Wang, Yu-Ligh Liou, Ying-Zi Liu, Hong-Hao Zhou and Yuan-Shan Zhu
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Jin Huang: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Guo Wang: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Jie Tang: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Wei Zhuang: Department of Cardiovascular & Thoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China
Li-Ping Wang: Department of Clinical Oncology, The First People’s Hospital of Chenzhou, Chenzhou 423000, Hunan, China
Yu-Ligh Liou: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Ying-Zi Liu: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Hong-Hao Zhou: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China
Yuan-Shan Zhu: Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410078, Hunan, China

IJERPH, 2017, vol. 14, issue 2, 1-12

Abstract: Hypermethylation of specific gene promoters is an important mechanism of carcinogenesis. A high frequency of promoter methylation of PAX1 and ZNF582 genes has been detected in cervical cancer. In the present study, we investigated the methylation status of PAX1 and ZNF582 genes in esophageal squamous cell carcinoma (ESCC) tissues. Tumor and paracancerous tissues were obtained from 14 ESCC patients. Genomic DNA was extracted from both tumor and paracancerous tissues, and the concentration of DNA were determined. DNA methylation analysis of PAX1 and ZNF582 genes was carried out using quantitative methylation-specific PCR. To assess the diagnostic performance of the two methylated genes for cancer detection, receiver operating characteristic (ROC) curves were generated. Sensitivities and specificities were tested at cut-offs obtained from the ROC curves. The methylation levels of both PAX1 and ZNF582 genes were significantly higher in tumor tissues compared to non-tumor paracancerous tissues. The methylation rates of PAX1 and ZNF582 in ESCC tumor and paracancerous tissues were 100% and 21.4% ( p = 0.006), 85.7% and 0% ( p < 0.001), respectively. The sensitivities and specificities of PAX1 and ZNF582 methylation for the detection of cancer were 100% and 85.7%, and 78.6% and 100%, respectively. The DNA methylation levels and frequencies of PAX1 and ZNF582 genes were markedly higher in ESCC tumor tissues compared to those in paracancerous tissues. Moreover, the conclusions were verified by using The Cancer Genome Atlas (TCGA) datasets. DNA methylation status of these two genes showed a relatively good sensitivity and specificity for the detection of ESCC tumors. This data suggests that DNA methylation testing holds a great promise for ESCC screening and warrants further prospective population-based studies.

Keywords: esophageal squamous cell carcinoma; DNA methylation; PAX1; ZNF582 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
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