Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas

Ana Stupin, Anita Cosic, Sanja Novak, Monika Vesel, Ivana Jukic, Brigita Popovic, Krunoslav Karalic, Zdenko Loncaric and Ines Drenjancevic
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Ana Stupin: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
Anita Cosic: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
Sanja Novak: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
Monika Vesel: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
Ivana Jukic: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia
Brigita Popovic: Department of Agroecology, Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
Krunoslav Karalic: Department of Agroecology, Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
Zdenko Loncaric: Department of Agroecology, Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia
Ines Drenjancevic: Department of Physiology and Immunology, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 10E, HR-31000 Osijek, Croatia

IJERPH, 2017, vol. 14, issue 6, 1-15

Abstract: This study aimed to determine whether low dietary Se content affects the function and mechanisms mediating the vascular relaxation of rat aortas, and to test the role of oxidative stress in observed differences. Male Sprague Dawley (SD) rats were maintained for 10 weeks on low Se (low-Se group; N = 20) or normal Se content (norm-Se group; N = 20) rat chow. Dose responses to acetylcholine (ACh; 10 ?9 –10 ?5 M) and the response to reduced pO 2 were tested in noradrenaline-precontracted aortic rings in the absence/presence of the nitric oxide synthase (NOS) inhibitor nitro- l -arginine methyl ester ( l -NAME), the cyclooxygenase 1 and 2 (COX-1, 2) inhibitor Indomethacin, and the antioxidative agent Tempol in tissue bath. mRNA expression of glutathione peroxidase 1 (GPx1), catalase (CAT), and Cu/Zn superoxide dismutase (SOD) was measured in rat aortas. Oxidative stress (Thiobarbituric Acid Reactive Substances; TBARS), antioxidative plasma capacity (ferric reducing ability of plasma assay; FRAP), and protein levels of GPx1 were measured in plasma and serum samples, respectively. Reduced ACh-induced relaxation (AChIR) (dominantly mediated by NO) in the low-Se group compared to the norm-Se group was restored by Tempol administration. Hypoxia-induced relaxation (HIR) (dominantly mediated by COX-1, 2), TBARS, and FRAP as well as GPx1 serum concentrations were similar between the groups. mRNA GPx1 expression in rat aortas was significantly decreased in the low-Se compared to the norm-Se group. These data suggest that low dietary Se content increases the local oxidative stress level, which subsequently affects the NO-mediated vascular response.

Keywords: selenium; vascular function; endothelium; oxidative stress; rats (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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