Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Elena Ojer-Usoz, David González and Ana Isabel Vitas
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Elena Ojer-Usoz: Department of Microbiology and Parasitology, University of Navarra, 31080 Pamplona, Spain
David González: Department of Microbiology and Parasitology, University of Navarra, 31080 Pamplona, Spain
Ana Isabel Vitas: Department of Microbiology and Parasitology, University of Navarra, 31080 Pamplona, Spain

IJERPH, 2017, vol. 14, issue 7, 1-13

Abstract: This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum ?-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of ?-lactamase genes ( bla CTX-M , bla SHV , bla TEM , bla OXA ). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent ?-lactamase genes were bla CTXM-14 (26%) and bla CTXM-1 (21.4%), followed by bla SHV-12 , bla CTX-M-15 and bla TEM-42 . MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers.

Keywords: ESBL-producing E. coli; ?-lactamase genes; clonal diversity; MLST (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2017
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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