Immunohistochemical Evaluation of Peri-Implant Soft Tissues around Machined and Direct Metal Laser Sintered (DMLS) Healing Abutments in Humans

Carlo Mangano, Francesco Guido Mangano, Jamil Awad Shibli, Leandro Amadeu Roth, Gianmaria D’ Addazio, Adriano Piattelli and Giovanna Iezzi
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Carlo Mangano: Department of Dental Sciences, Dental School, San Raffaele University, Milan 20132, Italy
Francesco Guido Mangano: Department of Medicine and Surgery, Dental School, Insubria University, Varese 21100, Italy
Jamil Awad Shibli: Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Guarulhos 743372, SP, Brazil
Leandro Amadeu Roth: Department of Periodontology and Oral Implantology, Dental Research Division, Guarulhos University, Guarulhos 743372, SP, Brazil
Gianmaria D’ Addazio: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio, Chieti 66100, Italy
Adriano Piattelli: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio, Chieti 66100, Italy
Giovanna Iezzi: Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio, Chieti 66100, Italy

IJERPH, 2018, vol. 15, issue 8, 1-18

Abstract: Background : Direct metal laser Sintering (DMLS) is an additive manufacturing technique that allows fabrication of dental implants and related components with a highly porous surface. To date, no human studies have investigated the soft tissue adhesion and presence of inflammatory infiltrate with porous DMLS healing abutments (HAs), nor have they compared these with the classic machined ones. Purpose : To evaluate the degree of cell adhesion (integrin expression) and the quantity/quality of inflammatory infiltrate, on HAs with different surfaces; full DMLS, full machined, and hybrid (half DMLS and half machined). Methods : Fifty implant patients were randomly assigned to receive one of these different Has: T1, full DMLS (11 subjects); T2, machined in the upper portion and DMLS in the lower one (10 subjects); T3, DMLS in the upper portion and machined in the lower one (19 subjects); T4, full machined (10 patients). Thirty days after placement, circular sections of soft tissues around HAs were retrieved for immunohistochemical evaluation. Results : With regard to the adhesion molecules, the samples showed different intensity of integrin expression, with a statistically significant difference ( p < 0.001) between T1 and the other groups. All the samples were positive for the different clusters related to the inflammatory infiltrate (T lymphocytes, CD3; B lymphocytes, CD20; and macrophages, CD68), but a lower infiltrate was found in T1, with statistically significant differences ( p < 0.001) among the groups. Conclusions: The HA surface seems to influence the degree of cell adhesion and the inflammatory infiltrate of the surrounding soft tissues.

Keywords: healing abutment; soft tissues; machined; direct metal laser sintering; humans (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2018
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