Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Pina Caputo, Maria Chiara Di Martino, Brunella Perfetto, Francesco Iovino and Giovanna Donnarumma
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Pina Caputo: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy
Maria Chiara Di Martino: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy
Brunella Perfetto: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy
Francesco Iovino: Division of General Surgery, Department of Cardiothoracic and Respiratory Science, University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy
Giovanna Donnarumma: Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy

IJERPH, 2018, vol. 15, issue 8, 1-11

Abstract: For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis , combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis , thus helping to establish an effective antibiotic therapy.

Keywords: Matrix-assisted; laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS); biofilm; Staphylococcus epidermidis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2018
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