Susceptibility Profiles of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) to Deltamethrin Reveal a Contrast between the Northern and the Southern Benin

Eric Tossou, Ghislain Tepa-Yotto, Ouorou K. Douro Kpindou, Ruth Sandeu, Benjamin Datinon, Francis Zeukeng, Romaric Akoton, Généviève M. Tchigossou, Innocent Djègbè, John Vontas, Thibaud Martin, Charles Wondji, Manuele Tamò, Aimé H. Bokonon-Ganta and Rousseau Djouaka
Additional contact information
Eric Tossou: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Ghislain Tepa-Yotto: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Ouorou K. Douro Kpindou: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Ruth Sandeu: Faculty of Science, Department of Biochemistry, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon
Benjamin Datinon: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Francis Zeukeng: Faculty of Science, Department of Biochemistry, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon
Romaric Akoton: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Généviève M. Tchigossou: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Innocent Djègbè: Life And Earth Sciences, National University of Science, Technology, Engineering and Mathematics, P.O. Box 2282 Abomey, Benin
John Vontas: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, N Plastira 100, 70013 Crete, Greece
Thibaud Martin: Cirad UR Hortsys, Université Montpellier, Campus international de Baillarguet, 34 398 Montpellier, France
Charles Wondji: Vector group, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK
Manuele Tamò: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin
Aimé H. Bokonon-Ganta: Direction of Plant Production, University of Abomey-Calavi, P.O. Box 526 Abomey-Calavi, Benin
Rousseau Djouaka: International Institute of Tropical Agriculture (IITA), 08 P.O. Tri-Postal, Box 0932 Cotonou, Benin

IJERPH, 2019, vol. 16, issue 11, 1-15

Abstract: Helicoverpa armigera is an indigenous species in Africa and has been reported in the destruction of several crops in Benin. Management of H. armigera pest is mainly focused on the use of synthetic pyrethroids, which may contribute to resistance selection. This study aimed to screen the susceptibility pattern of field populations of H. armigera to deltamethrin in Benin. Relevant information on the type of pesticides used by farmers were gathered through surveys. Collected samples of Helicoverpa (F 0 ) were reared to F 1 . F 0 were subjected to morphological speciation followed by a confirmation using restriction fragment length polymorphism coupled with a polymerase chain reaction (RFLP-PCR). F 1 (larvae) were used for insecticide susceptibility with deltamethrin alone and in the presence of the P450 inhibitor Piperonyl Butoxide (PBO). Deltamethrin and lambda-cyhalothrin were the most used pyrethroids in tomato and cotton farms respectively. All field-sampled Helicoverpa were found to be H. armigera . Susceptibility assays of H. armigera to deltamethrin revealed a high resistance pattern in cowpea (resistance factor (RF) = 2340), cotton (RF varying from 12 to 516) and tomato (RF=85) farms which is a concern for the control of this major polyphagous agricultural pest. There was a significant increase of mortality when deltamethrin insecticide was combined with piperonyl butoxide (PBO), suggesting the possible involvement of detoxification enzymes such as oxidase. This study highlights the presence of P450 induced metabolic resistance in H. armigera populations from diverse cropping systems in Benin. The recorded high levels of deltamethrin resistance in H. armigera is a concern for the control of this major agricultural pest in Benin as the country is currently embarking into economical expansion of cotton, vegetables and grain-legumes cropping systems.

Keywords: H. armigera; pyrethroid resistance; Benin (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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