Isolation, Screening, and Characterization of Antibiotic-Degrading Bacteria for Penicillin V Potassium (PVK) from Soil on a Pig Farm

Xuanjiang Yang, Miao Li, Panpan Guo, Hualong Li, Zelin Hu, Xianwang Liu and Qiang Zhang
Additional contact information
Xuanjiang Yang: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Miao Li: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Panpan Guo: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Hualong Li: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Zelin Hu: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Xianwang Liu: Institute of Intelligent Machinery, Hefei Institute of Material Sciences, Chinese Academy of Sciences, Hefei 230031, Anhui, China
Qiang Zhang: Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada

IJERPH, 2019, vol. 16, issue 12, 1-9

Abstract: (1) Background: Antibiotics are frequently used on farm animals, making animal husbandry a relatively large source of antibiotic pollution of the environment. The present study aims to isolate and acclimatize antibiotic-degrading bacterial strains for penicillin V potassium (PVK) from the contaminated soil of a pig farm. (2) Methods: Bacterial strains were isolated and acclimatized by continuous enrichment of cultures with PVK as the sole carbon source. The antibiotic susceptibility test, thiol mercury salt ultraviolet spectrophotometry (TMSUS), morphological observations, and 16S rDNA sequence analysis were used to identify and characterize the isolated strains. (3) Results: Four bacterial isolates (denoted as LM-1, LM-2, LM-3, LM-4) were obtained, and two of them (LM-1, LM-2) with the highest degradation rates were identified to belong to the same genera as Bacillus . These two isolates were found to be resistant to PVK antibiotic in an antibiotic sensitivity test. The TMSUS indicated that the strains LM-1 and LM-2 had good performance in PVK degradation (68% for LM-1, 66% for LM-2 in 48 h) when the initial PVK concentration was about 100 ?g/mL. (4) Conclusions: Two bacterial strains isolated from the soil on a pig farm are effective in degrading PVK and can be potentially used for bioremediation of PVK antibiotic-contaminated soils.

Keywords: penicillin; biodegradation; soil; pig farm; bacterial isolate; Bacillus (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/1660-4601/16/12/2166/pdf (application/pdf)
https://www.mdpi.com/1660-4601/16/12/2166/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:16:y:2019:i:12:p:2166-:d:241062

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().