Levels of Urinary Biomarkers of Oxidatively Generated Damage to DNA and RNA in Different Groups of Workers Compared to General Population

Giovanna Tranfo, Enrico Paci, Mariella Carrieri, Enrico Marchetti, Renata Sisto, Monica Gherardi, Francesca Costabile, Lisa Bauleo, Carla Ancona and Daniela Pigini
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Giovanna Tranfo: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
Enrico Paci: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
Mariella Carrieri: Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, via Giustiniani 2, 35128 Padova, Italy
Enrico Marchetti: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
Renata Sisto: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
Monica Gherardi: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy
Francesca Costabile: CNR-ISAC—Italian National Research Council, Institute of Atmospheric Science and Climate, via Fosso del Cavaliere 100, 00133 Rome, Italy
Lisa Bauleo: Department of Epidemiology, Lazio Regional Health Service, Via Cristoforo Colombo 112, 00147 Rome, Italy
Carla Ancona: Department of Epidemiology, Lazio Regional Health Service, Via Cristoforo Colombo 112, 00147 Rome, Italy
Daniela Pigini: Department of Occupational Medicine, Epidemiology, Occupational and Environmental Hygiene, INAIL Research, via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy

IJERPH, 2019, vol. 16, issue 16, 1-13

Abstract: (1) Background : The products of guanine oxidation in DNA and RNA excreted in urine are 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodGuo). Despite intra and inter-individual variability, it is possible to identify situations that significantly increase the levels of these compounds when comparing urinary concentrations of some workers to those of the general population. (2) Methods : urines from gasoline pump attendants (58 from Saudi Arabia and 102 from Italy), 24 workers of a fiberglass reinforced plastics plant, 17 painters and 6 divers were analyzed by HPLC/MS-MS. To test the individual variability, two subjects provided daily samples for one month, and 132 urine samples from the general population were analyzed. (3) Results : We summarized the results for each biomarker, and found the following were statistically higher than in the general population: 8-oxoGua in fiberglass and Italian gasoline workers; 8-oxodGuo in fiberglass and both Saudi Arabian and Italian gasoline workers; 8-oxoGuo in fiberglass workers, both Saudi Arabian and Italian gasoline workers, and painters after the working shift. (4) Conclusions : these results confirm that both 8-oxodGuo and 8-oxoGuo are valuable biomarkers for occupational exposures to dangerous chemicals and seem to suggest that 8-oxoGuo, related to RNA oxidation, is a suitable biomarker to evaluate short term, reversible effects of occupational exposures even within the health-based limit values.

Keywords: DNA and RNA oxidation; HPLC-MS/MS; biological monitoring; effect biomarkers; occupational exposure (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (3)

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