Co-Exposure to SiO 2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells

Maqusood Ahamed, Mohd Javed Akhtar and Hisham A. Alhadlaq
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Maqusood Ahamed: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11142, Saudi Arabia
Mohd Javed Akhtar: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11142, Saudi Arabia
Hisham A. Alhadlaq: King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11142, Saudi Arabia

IJERPH, 2019, vol. 16, issue 17, 1-14

Abstract: Widespread application of silica nanoparticles (nSiO 2 ) and ubiquitous metalloid arsenic (As) may increase their chances of co-exposure to human beings in daily life. Nonetheless, studies on combined effects of nSiO 2 and As in human cells are lacking. We investigated the co-exposure effects of nSiO 2 and As in human liver (HepG2) and human fibroblast (HT1080) cells. Results showed that nSiO 2 did not cause cytotoxicity. However, exposure of As caused oxidative stress and apoptosis in both types of cells. Interesting results were that co-exposure of a non-cytotoxic concentration of nSiO 2 significantly augmented the As induced toxicity in both cells. Intracellular level of As was higher in the co-exposure group (nSiO 2 + As) than the As group alone, suggesting that nSiO 2 facilitates the cellular uptake of As. Co-exposure of nSiO 2 and As potentiated oxidative stress indicated by pro-oxidants generation (reactive oxygen species, hydrogen peroxide and lipid peroxidation) and antioxidants depletion (glutathione level, and glutathione reductase, superoxide dismutase and catalase activities). In addition, co-exposure of nSiO 2 and As also potentiated mitochondria-mediated apoptosis suggested by increased expression of p53 , bax , caspase-3 and caspase-9 genes (pro-apoptotic) and decreased expression of bcl-2 gene (anti-apoptotic) along with depleted mitochondrial membrane potential. To the best of our knowledge, this is the first study showing that co-exposure of nSiO 2 and As induced augmentation of oxidative stress and mitochondria-mediated apoptosis in HepG2 and HT1080 cells. Hence, careful attention is required for human health assessment following combined exposure to nSiO 2 and As.

Keywords: combined toxicity; SiO 2 nanoparticles; arsenic; human health; oxidative stress; apoptosis (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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