The Role of the Activation of the TRPV1 Receptor and of Nitric Oxide in Changes in Endothelial and Cardiac Function and Biomarker Levels in Hypertensive Rats

Juan Carlos Torres-Narváez, Israel Pérez-Torres, Vicente Castrejón-Téllez, Elvira Varela-López, Víctor Hugo Oidor-Chan, Verónica Guarner-Lans, Álvaro Vargas-González, Raúl Martínez-Memije, Pedro Flores-Chávez, Etzna Zizith Cervantes-Yañez, Claudia Angélica Soto-Peredo, Gustavo Pastelín-Hernández and Leonardo del Valle-Mondragón
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Juan Carlos Torres-Narváez: Departamento de Farmacología “Dr. Rafael Méndez Martínez”, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Israel Pérez-Torres: Departamento de Patología, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Vicente Castrejón-Téllez: Departamento de Fisiología Celular, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Elvira Varela-López: Laboratorio de Cardiología Traslacional, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Víctor Hugo Oidor-Chan: Departamento de Farmacología “Dr. Rafael Méndez Martínez”, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Verónica Guarner-Lans: Departamento de Fisiología Celular, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Álvaro Vargas-González: Departamento de Fisiología Celular, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Raúl Martínez-Memije: Departamento de Instrumentación Electromecánica, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Pedro Flores-Chávez: Departamento de Instrumentación Electromecánica, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Etzna Zizith Cervantes-Yañez: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, 04960 Coyoacán, CDMX, Mexico
Claudia Angélica Soto-Peredo: Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, 04960 Coyoacán, CDMX, Mexico
Gustavo Pastelín-Hernández: Departamento de Farmacología “Dr. Rafael Méndez Martínez”, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico
Leonardo del Valle-Mondragón: Departamento de Farmacología “Dr. Rafael Méndez Martínez”, Instituto Nacional de Cardiología “Ignacio Chávez”, 14080 Tlalpan, CDMX, Mexico

IJERPH, 2019, vol. 16, issue 19, 1-16

Abstract: The purpose of the present study was to analyze the actions of transient receptor potential vanilloid type 1 (TRPV1) agonist capsaicin (CS) and of its antagonist capsazepine (CZ), on cardiac function as well as endothelial biomarkers and some parameters related with nitric oxide (NO) release in L -N G -nitroarginine methyl ester ( L -NAME)-induced hypertensive rats. NO has been implicated in the pathophysiology of systemic arterial hypertension (SAHT). We analyzed the levels of nitric oxide (NO), tetrahydrobiopterin (BH4), malondialdehyde (MDA), total antioxidant capacity (TAC), cyclic guanosin monophosphate (cGMP), phosphodiesterase-3 (PDE-3), and the expression of endothelial nitric oxide synthase (eNOS), guanosine triphosphate cyclohydrolase 1 (GTPCH-1), protein kinase B (AKT), and TRPV1 in serum and cardiac tissue of normotensive (118±3 mmHg) and hypertensive (H) rats (165 ± 4 mmHg). Cardiac mechanical performance (CMP) was calculated and NO was quantified in the coronary effluent in the Langendorff isolated heart model. In hypertensive rats capsaicin increased the levels of NO, BH4, cGMP, and TAC, and reduced PDE-3 and MDA. Expressions of eNOS, GTPCH-1, and TRPV1 were increased, while AKT was decreased. Capsazepine diminished these effects. In the hypertensive heart, CMP improved with the CS treatment. In conclusion, the activation of TRPV1 in H rats may be an alternative mechanism for the improvement of cardiac function and systemic levels of biomarkers related to the bioavailability of NO.

Keywords: nitric oxide; TRPV1; hypertension; capsaicin; capsazepine; L -NAME (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:16:y:2019:i:19:p:3576-:d:270389

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