Effects of Bacillus thuringiensis HC-2 Combined with Biochar on the Growth and Cd and Pb Accumulation of Radish in a Heavy Metal-Contaminated Farmland under Field Conditions

Zigang Li, Peng Wang, Xiaoyu Yue, Jingtao Wang, Baozeng Ren, Lingbo Qu and Hui Han
Additional contact information
Zigang Li: School of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Peng Wang: School of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Xiaoyu Yue: School of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China
Jingtao Wang: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, China
Baozeng Ren: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, China
Lingbo Qu: State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, China
Hui Han: Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China

IJERPH, 2019, vol. 16, issue 19, 1-15

Abstract: The objective of this study was to explore the effect of heavy metal-resistant bacteria and biochar (BC) on reducing heavy metal accumulation in vegetables and the underlying mechanism. We tested Bacillus thuringiensis HC-2, BC, and BC+HC-2 for their ability to immobilize Cd and Pb in culture solution. We also studied the effects of these treatments on the dry weight and Cd and Pb uptake of radish in metal-contaminated soils under field conditions and the underlying mechanism. Treatment with HC-2, BC, and BC+HC-2 significantly reduced the water-soluble Cd (34–56%) and Pb (31–54%) concentrations and increased the pH and NH 4 + concentration in solution compared with their vales in a control. These treatments significantly increased the dry weight of radish roots (18.4–22.8%) and leaves (37.8–39.9%) and decreased Cd (28–94%) and Pb (22–63%) content in the radish roots compared with the control. Treatment with HC-2, BC, and BC+HC-2 also significantly increased the pH, organic matter content, NH 4 + content, and NH 4 + /NO 3 ? ratio of rhizosphere soils, and decreased the DTPA-extractable Cd (37–58%) and Pb (26–42%) contents in rhizosphere soils of radish. Furthermore, BC+HC-2 had higher ability than the other two treatments to protect radish against Cd and Pb toxicity and increased radish biomass. Therefore, Bacillus thuringiensis HC-2 combined with biochar can ensure vegetable safety in situ for the bioremediation of heavy metal-polluted farmland.

Keywords: Bacillus thuringiensis; plant growth-promoting bacteria; biochar; heavy metal; radish (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.mdpi.com/1660-4601/16/19/3676/pdf (application/pdf)
https://www.mdpi.com/1660-4601/16/19/3676/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:16:y:2019:i:19:p:3676-:d:272226

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().