Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats

Ren Lin, Yue Jia, Fengjuan Wu, Yuan Meng, Qi Sun and Lihong Jia
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Ren Lin: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China
Yue Jia: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China
Fengjuan Wu: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China
Yuan Meng: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China
Qi Sun: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China
Lihong Jia: Department of Child and Adolescent Health, School of Public Health, China Medical University, Shenyang 110122, China

IJERPH, 2019, vol. 16, issue 21, 1-13

Abstract: The aim of this study was to investigate whether combined exposure to fructose and bisphenol A (BPA) has a synergistic effect on abnormal lipid metabolism in the liver of developmental male rats and its possible mechanism. Fifty weaned male Wistar rats were divided into five groups: the control, 13% fructose, 20% fructose, 1 µg/mL BPA, and 13% fructose + 1 µg/mL BPA (combined exposure). Rats were exposed to fructose and/or BPA through drinking water for eight weeks. Genes or proteins regulating lipid metabolism include sterol regulatory element binding protein 1 (SREBP1), adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), zinc α 2 glycoprotein (ZAG) and estrogen receptor α (ERα), and the expression of proteins regulating inflammatory response, such as TLR4 and NF-κB, were determined. Serum total cholesterol (T-CHO), triglyceride (TG), low, high density lipoprotein cholesterol (LDL-C, HDL-C), blood glucose, insulin, IL-17 and TNF-α levels were also measured. Liver tissue morphology was observed by H&E staining. The results showed that the levels of gene and protein catalyzing lipogenesis were increased (SREBP1, ACC1 and FAS), while those catalyzing lipolysis were decreased (ATGL, HSL and ZAG), accompanied by dyslipidemia, insulin resistance and hepatic fat accumulation, and there were higher expression of TLR4 and NF-κB protein and lower expression of ERα protein in liver, and increased serum IL-17 and TNF-α levels in fructose and/or BPA exposed rats compared with controls. Moreover, the above indicators were more serious in combined exposure group than in single exposure group. Therefore, abnormal lipid metabolism in the liver of developmental rats could be exacerbated by combined exposed to fructose and BPA.

Keywords: fructose; bisphenol A; lipid metabolism; inflammatory response (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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