Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1

Cristobal Espinosa Ruiz, Simona Manuguerra, Alberto Cuesta, Andrea Santulli and Concetta M. Messina
Additional contact information
Cristobal Espinosa Ruiz: Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy
Simona Manuguerra: Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy
Alberto Cuesta: Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Spain
Andrea Santulli: Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy
Concetta M. Messina: Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy

IJERPH, 2019, vol. 16, issue 3, 1-12

Abstract: The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, the quantification of proteins related to cell cycle and DNA repair (p53), cell proliferation (extracellular signal–regulated kinase 1 (ERK1)), energetic restriction (hypoxia-inducible factor 1 (HIF1)), and redox status (Nuclear factor erythroid 2–related factor 2 (NRF2)) was also determined after prolonged exposure (7–15 days) by immunoblotting. Our results demonstrated that rising concentrations of PBDEs exposure-induced oxidative stress, and that this event modulates different cell pathways related to cell cycle, cell signaling, and energetic balance in the long term, indicating the negative impact of sub-lethal dose exposure to cell homeostasis.

Keywords: PBDE-209; Sparus aurata fibroblast; oxidative stress; biomarkers (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/16/3/474/pdf (application/pdf)
https://www.mdpi.com/1660-4601/16/3/474/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:16:y:2019:i:3:p:474-:d:203824

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().