Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation

Shiyue Liu, Yi Zhang, Ping Zeng, Heli Wang, Yonghui Song and Juan Li
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Shiyue Liu: School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Road, Haidian District, Beijing 100083, China
Yi Zhang: Fudan University, 2005 Songhu Road, Yangpu District, Shanghai 200438, China
Ping Zeng: Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China
Heli Wang: School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Road, Haidian District, Beijing 100083, China
Yonghui Song: Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China
Juan Li: Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China

IJERPH, 2019, vol. 16, issue 4, 1-12

Abstract: Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h) −1 , Km 66.68 ± 8.95 mg L −1 , and Ki 43.16 ± 5.92 mg L −1 , with an R 2 of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L −1 could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes.

Keywords: Berberine; Sphingopyxis sp., 16S rDNA; degradation kinetics (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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