Sulforaphane Cannot Protect Human Fibroblasts From Repeated, Short and Sublethal Treatments with Hydrogen Peroxide

Maria Chiara Lionetti, Federico Mutti, Erica Soldati, Maria Rita Fumagalli, Valentina Coccé, Graziano Colombo, Emanuela Astori, Alessandro Miani, Aldo Milzani, Isabella Dalle-Donne, Emilio Ciusani, Giulio Costantini and Caterina A. M. La Porta
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Maria Chiara Lionetti: Center for Complexity and Biosystems, Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133 Milano, Italy
Federico Mutti: Center for Complexity and Biosystems, Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133 Milano, Italy
Erica Soldati: Center for Complexity and Biosystems, Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133 Milano, Italy
Maria Rita Fumagalli: Center for Complexity and Biosystems, Department of Physics, University of Milan, via Celoria 16, 20133 Milano, Italy
Valentina Coccé: Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via Pascal 36, 20133 Milano, Italy
Graziano Colombo: Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy
Emanuela Astori: Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy
Alessandro Miani: Department of Environmental Science and Policy, University of Milan, via Celoria 10, 20133 Milano, Italy
Aldo Milzani: Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy
Isabella Dalle-Donne: Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy
Emilio Ciusani: Fondazione IRCCS Istituto Neurologico C. Besta, Via Celoria 11, 20133 Milano, Italy
Giulio Costantini: Center for Complexity and Biosystems, Department of Physics, University of Milan, via Celoria 16, 20133 Milano, Italy
Caterina A. M. La Porta: Center for Complexity and Biosystems, Department of Environmental Science and Policy, University of Milan, via Celoria 26, 20133 Milano, Italy

IJERPH, 2019, vol. 16, issue 4, 1-13

Abstract: A delicate balance of reactive oxygen species (ROS) exists inside the cell: when the mechanisms that control the level of ROS fail, the cell is in an oxidative stress state, a condition that can accelerate aging processes. To contrast the pro-aging effect of ROS, the supplementation of antioxidants has been recently proposed. Sulforaphane (SFN) is an isothiocyanate isolated from Brassica plants that has been shown to modulate many critical factors inside the cells helping to counteract aging processes. In the present work, we exposed human dermal fibroblast to short, sublethal and repeated treatments with hydrogen peroxide for eight days, without or in combination with low concentration of SFN. Hydrogen peroxide treatments did not affect the oxidative status of the cells, without any significant change of the intracellular ROS levels or the number of mitochondria or thiols in total proteins. However, our regime promoted cell cycle progression and cell viability, increased the anti-apoptotic factor survivin and increased DNA damage, measured as number of foci positive for γ -H2AX. On the other hand, the treatment with SFN alone seemed to exert a protective effect, increasing the level of p53, which can block the expansion of possible DNA damaged cells. However, continued exposure to SFN at this concentration could not protect the cells from stress induced by hydrogen peroxide.

Keywords: oxidative stress; sulforaphane; fibrobalsts; p53 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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