Molecular Analysis of HLA-G in Women with High-Risk Pregnancy and Their Partners with Regard to Possible Complications

Olimpia Sipak, Aleksandra Rył, Anna Grzywacz, Maria Laszczyńska, Sławomir Szymański, Beata Karakiewicz, Iwona Rotter and Cezary Cybulski
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Olimpia Sipak: Department of Obstetrics and Pathology of Pregnancy, Pomeranian Medical University in Szczecin, 71-210 Szczecin, ul. Żołnierska 48, Poland
Aleksandra Rył: Department of Medical Rehabilitation and Clinical Physiotherapy, Pomeranian Medical University in Szczecin, 71-210 Szczecin, ul. Żołnierska 48, Poland
Anna Grzywacz: Independent Laboratory of Health Promotion, Pomeranian Medical University in Szczecin, 70-103 Szczecin, ul. Gen. Dezyderego Chłapowskiego 1, Poland
Maria Laszczyńska: Department of Histology and Developmental Biology, Pomeranian Medical University in Szczecin, 71-210 Szczecin, ul. Żołnierska 48, Poland
Sławomir Szymański: Department of Obstetrics and Pathology of Pregnancy, Pomeranian Medical University in Szczecin, 71-210 Szczecin, ul. Żołnierska 48, Poland
Beata Karakiewicz: Department of Public Health, Pomeranian Medical University in Szczecin; 71-210 Szczecin, ul. Żołnierska 48, Poland
Iwona Rotter: Department of Medical Rehabilitation and Clinical Physiotherapy, Pomeranian Medical University in Szczecin, 71-210 Szczecin, ul. Żołnierska 48, Poland
Cezary Cybulski: Department of Genetics and Pathology, Pomeranian Medical University in Szczecin, 71-252 Szczecin, ul. Unii Lubelskiej 1, Poland

IJERPH, 2019, vol. 16, issue 6, 1-13

Abstract: The understanding of the molecular and biochemical characteristics of the human leukocyte antigen-G ( HLA-G ) is important because of the diverse influence of this antigen’s polymorphisms on the course of a pregnancy. The aim of our study was to assess how the variation of the HLA-G allele and the HLA-G 14-bp ins/del polymorphism influence predisposition to a complicated pregnancy. The clinical material consisted of parental pairs with complicated pregnancies (210 women; 190 men). The control group included parental pairs without complications during pregnancy (89 women; 86 men). The study involved isolation of genome DNA from peripheral blood leukocytes, sequencing, and analysis of the 14-bp ins/del polymorphism in the 3?-untranslated region (3?-UTR) of the HLA-G gene based on polymerase chain reaction (PCR). The most common HLA-G allele in the group of women with complicated pregnancies was the HLA-G 10101 allele. There were no statistically significant differences in the frequencies of the 14-bp ins/del polymorphism in the 3?UTR of the HLA-G gene between the groups. Our results suggest that the risk of complications in pregnancy is influenced by the HLA-G 10101 , HLA-G 10108 , and HLA-G 10106 alleles and is not influenced by the 14-bp ins/del polymorphism in the 3?UTR of the HLA-G gene.

Keywords: HLA-G; allele; haplotype; pregnancy (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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