Oxidative Stress Effects of Soluble Sulfide on Human Hepatocyte Cell Line LO2

Ying Shao, Zhongli Chen and Lingling Wu
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Ying Shao: Institute for Environmental Research (Bio V), RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany
Zhongli Chen: Key Laboratory of the Three Gorges Reservoir Eco-environment, Ministry of Education, Chongqing University, Chongqing 400045, China
Lingling Wu: Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Shanghai 200092, China

IJERPH, 2019, vol. 16, issue 9, 1-11

Abstract: Soluble sulfide is well known for its toxicity and corrosion for hundreds of years. However, recent studies have demonstrated that hydrogen sulfide (H 2 S)—a novel gasotransmitter—supports a critical role during neuromodulation, cell proliferation, and cardioprotection for organisms. In particular, soluble sulfide plays multifaceted signaling functions in mammals during oxidative stress processes. However, the specific molecular regulation of soluble sulfide during oxidative stress remains unclear. In this study, Na 2 S was implemented as a soluble sulfide donor to expose LO2 cells. The 3-(4,5-dimethylthiazolyl-2),-2,5-diphenyltetrazolium bromide (MTT) assay, hydroxyl radical assay, superoxide dismutase (SOD) assay, and glutathione peroxidase (GSH-PX) assay were applied to analyze cytotoxicity, hydroxyl radical levels, SOD and GSH-Px activities, respectively. Soluble sulfide at a concentration 0.01–1.0 mM/L resulted in a marked and concentration-dependent reduction of LO2 cell viability. At low concentrations, sulfide solutions increased SOD activity and GSH-Px activity of LO2 after 24 h exposure, exhibiting a clear hormesis-effect and indicating the protective ability of soluble sulfide against oxidative stress. The decline in SOD and GSH-Px and the increase in hydroxyl radical (0.08–1.0 mM/L) suggested that oxidative damage could be a possible mechanism for sulfide-induced cytotoxicity.

Keywords: soluble sulfide; oxidative stress; cytotoxicity; superoxide dismutase (SOD) activity; glutathione peroxidase (GSH-Px) activity (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2019
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