Caries Increment and Salivary Microbiome during University Life: A Prospective Cohort Study

Yoko Uchida-Fukuhara, Daisuke Ekuni, Md Monirul Islam, Kota Kataoka, Ayano Taniguchi-Tabata, Daiki Fukuhara, Naoki Toyama, Terumasa Kobayashi, Kohei Fujimori, Nanami Sawada, Yoshiaki Iwasaki and Manabu Morita
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Yoko Uchida-Fukuhara: Department of Preventive Dentistry, Okayama University Hospital, Okayama 700-8558, Japan
Daisuke Ekuni: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Md Monirul Islam: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Kota Kataoka: Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan
Ayano Taniguchi-Tabata: Department of Preventive Dentistry, Okayama University Hospital, Okayama 700-8558, Japan
Daiki Fukuhara: Department of Preventive Dentistry, Okayama University Hospital, Okayama 700-8558, Japan
Naoki Toyama: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Terumasa Kobayashi: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Kohei Fujimori: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Nanami Sawada: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan
Yoshiaki Iwasaki: Health Service Center, Okayama University, Okayama 700-8530, Japan
Manabu Morita: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan

IJERPH, 2020, vol. 17, issue 10, 1-12

Abstract: The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group ( n = 14) was similar to that in the non-increased group ( n = 41). Relative abundances of the family Prevotellaceae ( p = 0.007) and genera Alloprevotella ( p = 0.007) and Dialister ( p = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.

Keywords: salivary microbiome; sequence analysis; young adult; dental caries; saliva; oral health (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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