Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Yuqin Duan, Wei Wu, Qiuzi Zhao, Sihua Liu, Hongyun Liu, Mengqian Huang, Tao Wang, Mifang Liang and Zhiyun Wang
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Yuqin Duan: School of Life Sciences, Tianjin University, Tianjin 300072, China
Wei Wu: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100000, China
Qiuzi Zhao: School of Life Sciences, Tianjin University, Tianjin 300072, China
Sihua Liu: School of Life Sciences, Tianjin University, Tianjin 300072, China
Hongyun Liu: School of Life Sciences, Tianjin University, Tianjin 300072, China
Mengqian Huang: School of Life Sciences, Tianjin University, Tianjin 300072, China
Tao Wang: School of Life Sciences, Tianjin University, Tianjin 300072, China
Mifang Liang: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100000, China
Zhiyun Wang: School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China

IJERPH, 2020, vol. 17, issue 12, 1-15

Abstract: As humans and climate change continue to alter the landscape, novel disease risk scenarios have emerged. Sever fever with thrombocytopenia syndrome (SFTS), an emerging tick-borne infectious disease first discovered in rural areas of central China in 2009, is caused by a novel bunyavirus (SFTSV). The potential for SFTS to spread to other countries in combination with its high fatality rate, possible human-to-human transmission, and extensive prevalence among residents and domesticated animals in endemic regions make the disease a severe threat to public health. Because of the lack of preventive vaccines or useful antiviral drugs, diagnosis of SFTS is the key to prevention and control of the SFTSV infection. The development of serological detection methods will greatly improve our understanding of SFTSV ecology and host tropism. We describe a highly sensitive protein detection method based on gold nanoparticles (AuNPs) and enzyme-linked immunosorbent assay (ELISA)—AuNP-based ELISA. The optical sensitivity enhancement of this method is due to the high loading efficiency of AuNPs to McAb. This enhances the concentration of the HRP enzyme in each immune sandwich structure. The detection limit of this method to the nucleocapsid protein (NP) of SFTSV was 0.9 pg mL −1 with good specificity and reproducibility. The sensitivity of AuNP-based ELISA was higher than that of traditional ELISA and was comparable to real-time quantitative polymerase chain reaction (qRT-PCR). The probes are stable for 120 days at 4 °C. This can be applied to diagnosis and hopefully can be developed into a commercial ELISA kit. The ultrasensitive detection of SFTSV will increase our understanding of the distribution and spread of SFTSV, thus helping to monitor the changes in tick-borne pathogen SFTSV risk in the environment.

Keywords: SFTSV; nucleocapsid protein; gold nanoparticles; sandwich enzyme-linked immunosorbent assay (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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