The Effects of Long-Term Immunosuppressive Therapies on the Structure of the Rat Prostate

Marta Grabowska, Maria Laszczyńska, Karolina Kędzierska-Kapuza, Andrzej Kram, Kamil Gill and Małgorzata Piasecka
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Marta Grabowska: Department of Histology and Developmental Biology, Pomeranian Medical University, 71-210 Szczecin, Poland
Maria Laszczyńska: Department of Histology and Developmental Biology, Pomeranian Medical University, 71-210 Szczecin, Poland
Karolina Kędzierska-Kapuza: Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland
Andrzej Kram: Department of Pathology, West Pomeranian Oncology Center, 71-730 Szczecin, Poland
Kamil Gill: Department of Histology and Developmental Biology, Pomeranian Medical University, 71-210 Szczecin, Poland
Małgorzata Piasecka: Department of Histology and Developmental Biology, Pomeranian Medical University, 71-210 Szczecin, Poland

IJERPH, 2020, vol. 17, issue 12, 1-18

Abstract: Background: Little is known about the overall impact of immunosuppressive drugs on the prostate. The study aimed to determine the impact of different protocols of immunosuppressive treatment on the structure of the rat ventral prostate. Methods : For 6 months, 48 male Wistar rats received immunosuppressive drugs: cyclosporin A, tacrolimus, mycophenolate mofetil, rapamycin, and prednisone, according to three-drug protocols. Light and transmission electron microscopic studies, and quantitative evaluation of immunohistochemical expression of selected intermediate filaments, CD117 + mast cells, and CD138 + plasma cells were performed in the rat ventral prostate. Results: In all experimental groups, acini focal hyperplasia, changes to the ultrastructure of the glandular epithelium, changes in the expression of cytokeratins and desmin, and numerous mast and plasma cells in the prostate stroma were found. In cyclosporine-A-based groups, atrophy and numerous intracellular vacuoles were observed. In groups where a three-drug treatment was replaced with rapamycin, morphological alterations were less severe compared to those without conversion. Conclusions: In the rat ventral prostate, (1) immunosuppressive protocols affect the morphology and immunohistochemical expression of intermediate filaments, (2) morphological alterations, expression, and localization of selected proteins are not connected with adenocarcinoma development, and (3) conversion of the treatment to rapamycin may prevent hyperplastic abnormalities.

Keywords: Immunosuppressants; rat; ventral prostate; intermediate filaments; mast cells; plasma cells; ultrastructure; rapamycin (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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