Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

Hana Mlejnkova, Katerina Sovova, Petra Vasickova, Vera Ocenaskova, Lucie Jasikova and Eva Juranova
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Hana Mlejnkova: T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic
Katerina Sovova: T. G. Masaryk Water Research Institute, Public Research Institution, Brno Branch, Mojmirovo namesti 16, 612 00 Brno, Czech Republic
Petra Vasickova: Veterinary Research Institute, Public Research Institution, Hudcova 296/70, 621 00 Brno, Czech Republic
Vera Ocenaskova: T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic
Lucie Jasikova: T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic
Eva Juranova: T. G. Masaryk Water Research Institute, Public Research Institution, Podbabska 2582/30, 160 00 Prague, Czech Republic

IJERPH, 2020, vol. 17, issue 15, 1-9

Abstract: The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

Keywords: wastewater-based epidemiology; early warning system; virus; coronavirus; SARS-CoV-2; wastewater; epidemic; RT-qPCR (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (4)

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