Exercise- and Cold-Induced Human PGC-1? mRNA Isoform Specific Responses

Camille Larson, Megan Opichka, Mark L. McGlynn, Christopher W. Collins and Dustin Slivka
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Camille Larson: School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
Megan Opichka: School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
Mark L. McGlynn: School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
Christopher W. Collins: School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA
Dustin Slivka: School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA

IJERPH, 2020, vol. 17, issue 16, 1-10

Abstract: Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α , the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α , which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a , PGC-1α-b , and PGC-1α-c . Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention ( p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α ( p = 0.028) and its truncated isoform, NT-PGC-1α ( p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response.

Keywords: RT-qPCR; transcription; truncated; alternate promoter; primary promoter (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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