Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17?-Estradiol in Porcine Ovary and Thyroid Homogenates

Aleksandra Rynkowska, Jan Stępniak and Małgorzata Karbownik-Lewińska
Additional contact information
Aleksandra Rynkowska: Department of Oncological Endocrinology, Medical University of Łódź, 90-752 Łódź, Poland
Jan Stępniak: Department of Oncological Endocrinology, Medical University of Łódź, 90-752 Łódź, Poland
Małgorzata Karbownik-Lewińska: Department of Oncological Endocrinology, Medical University of Łódź, 90-752 Łódź, Poland

IJERPH, 2020, vol. 17, issue 18, 1-9

Abstract: The Fenton reaction (Fe 2+ +H 2 O 2 →Fe 3+ + • OH+OH - ) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H 2 O 2 ) are in excess. This study aims at comparing Fe 2+ +H 2 O 2 -induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17β-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO 4 +H 2 O 2 with/without 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO 4 concentrations (2400, 1200, 600, 300, 150 µM); (2nd) FeSO 4 (2400, 1200, 600, 300, 150 µM)+H 2 O 2 with/without 17β-estradiol; three experiments were performed with three highest 17β-estradiol concentrations; (3rd) FeSO 4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 µM)+H 2 O 2 (5 mM). LPO level [MDA+4-HDA/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe 2+ concentrations (2400, 1200, 1100 µM). Exogenous 17β-estradiol (1 mM, 100, and 10 µM) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17β-estradiol activity. The therapeutic effect of 17β-estradiol against iron overload consequences should be considered with relation to both tissues.

Keywords: 17?-estradiol; Fenton reaction; ferrous; lipid peroxidation; oxidative damage (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/17/18/6841/pdf (application/pdf)
https://www.mdpi.com/1660-4601/17/18/6841/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:17:y:2020:i:18:p:6841-:d:415919

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().