Pseudomonas fluorescens: A Bioaugmentation Strategy for Oil-Contaminated and Nutrient-Poor Soil

Eduardo Jahir Gutiérrez, María del Rosario Abraham, Juan Carlos Baltazar, Guadalupe Vázquez, Eladio Delgadillo and David Tirado
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Eduardo Jahir Gutiérrez: Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Campeche, Av. Agustín Melgar S/N, Buena Vista, Campeche 24039, Mexico
María del Rosario Abraham: División de Ciencias de la Vida, Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Hacienda El Copal Km 9, Carretera Irapuato-Silao; AP 311, Gto. Irapuato 36500, Mexico
Juan Carlos Baltazar: División de Ingenierías, Departamento de Ingeniería en Minas, Universidad de Guanajuato, Metalurgia y Geología, Ex Had. De San Matias S/N. Col. San Javier, Gto. Guanajuato 36020, Mexico
Guadalupe Vázquez: División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico
Eladio Delgadillo: División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico
David Tirado: División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico

IJERPH, 2020, vol. 17, issue 19, 1-14

Abstract: Bioremediation technology is one of the most profitable and sustainable strategies for remediating soils contaminated with hydrocarbons. This study focuses on assessing the influence of biostimulation and bioaugmentation with Pseudomonas fluorescens to contribute to the removal of total petroleum hydrocarbons (TPHs) of a soil. Laboratory studies were carried out (measurements of emitted CO 2 , surface tension, and residual TPH) to select the best bioaugmentation and biostimulation treatment. The sources of C, N, and P were glucose–yeast extract, NH 4 Cl–NaNO 3 , and K 2 HPO 4 –K 3 PO 4 , respectively. The effect of culture conditions on the reduction of TPH and respiratory activity was evaluated through a factorial design, 2 3 , in a solid culture system. After 80 days of incubation, it was observed that treatments of yeast extract–NH 4 Cl–K 2 HPO 4 (Y4) and glucose–NaNO 3 –K 3 PO 4 (Y5) presented a higher level of TPH removal (20.91% and 20.00% degradation of TPH, respectively). Biostimulation favors the production of biosurfactants, indirectly measured by the change in surface tension in the soil extracts. The treatments Y4 and Y5 showed a lower change value of the surface tension (23.15 and 23.30 mN·m −1 at 25 °C). A positive correlation was determined between the change in surface tension and the removal of TPH; hence there was a contribution of the biosurfactants produced to the removal of hydrocarbons.

Keywords: TPH; Pseudomonas fluorescens; biodegradation; bioavailability; biosurfactant; surface tension (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations: View citations in EconPapers (2)

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