Analysis of Human Papillomavirus (HPV) 16 Variants Associated with Cervical Infection in Italian Women

Marianna Martinelli, Chiara Villa, Giovanni Sotgiu, Narcisa Muresu, Federica Perdoni, Rosario Musumeci, Romina Combi, Antonio Cossu, Andrea Piana and Clementina Cocuzza
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Marianna Martinelli: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Chiara Villa: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Giovanni Sotgiu: Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Narcisa Muresu: Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Federica Perdoni: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Rosario Musumeci: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Romina Combi: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy
Antonio Cossu: Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Andrea Piana: Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy
Clementina Cocuzza: Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy

IJERPH, 2020, vol. 17, issue 1, 1-10

Abstract: This study aims to evaluate HPV16 variants distribution in a population of Italian women living in two different regions (Lombardy and Sardinia) by sequence analyses of HPV16-positive cervical samples, in order to reconstruct the phylogenetic relationship among variants to identify the currently circulating lineages. Analyses were conducted starting from DNA isolated from 67 HPV16-positive cervical samples collected from two different Italian centres (31 from Lombardy and 36 from Sardinia) of women with normal and abnormal cervical cytology. The entire long control region (LCR) and 300 nt of the E6 gene was sequenced to identify intra-type variants. Sequence comparison and phylogenetic analysis were made using a distance-based neighbour joining method (NJ) and Kimura two-parameter model. Data obtained reported that Italian sequences mainly belonged to the European lineage, in particular sublineage A2. Only five sequences clustered in non-European branches: two in North American lineage (sublineage D1), two in African-1 (sublineage B1) and one in African-2. A new 27 nucleotide duplication in the central segment of the LCR region was found in a sequence obtained from a sample isolated in Sardinia. A predominance of European variants was detected, with some degree of variability among the studied HPV16 strains. This study contributes to the implementation of data regarding the molecular epidemiology of HPV16 variants.

Keywords: HPV genotypes; HPV variants; LCR mutations; HPV16 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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