Development of a Rapid and Eco-Friendly UHPLC Analytical Method for the Detection of Histamine in Fish Products

Antonello Cicero, Francesco Giuseppe Galluzzo, Gaetano Cammilleri, Andrea Pulvirenti, Giuseppe Giangrosso, Andrea Macaluso, Antonio Vella and Vincenzo Ferrantelli
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Antonello Cicero: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Francesco Giuseppe Galluzzo: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Gaetano Cammilleri: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Andrea Pulvirenti: Dipartimento di Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Via Università 4, 41121 Modena, Italy
Giuseppe Giangrosso: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Andrea Macaluso: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Antonio Vella: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy
Vincenzo Ferrantelli: Istituto Zooprofilattico Sperimentale della Sicilia ‘A. Mirri’, via Gino Marinuzzi, 3, 90129 Palermo, Italy

IJERPH, 2020, vol. 17, issue 20, 1-11

Abstract: We developed, validated, and confirmed with proficiency tests a fast ultra-high-performance liquid chromatography with diode array detector (UHPLC-DAD) method to determine histamine in fish and fishery products. The proposed method consists of two successive solid–liquid extractions: one with a dilute solution of perchloric acid (6%) and the second only with water. The instrumental analysis with UHPLC provides a very fast run time (only 6 min) with a retention time of approximately 4 min, a limit of quantification (LOQ) of 7.2 mg kg −1 , a limit of detection (LOD) of 2.2 mg kg −1 , a recovery around 100%, a relative standard deviation (RSD%) between 0.5 and 1.4, and an r 2 of calibration curve equal to 0.9995. The method detected optimal values of the validation parameters and required a limited number of reagents in comparison to other methods reported in the literature. Furthermore, the method could detect histamine in a very short time compared with other methods. This method, in addition to being validated, precise, specific, and accurate, avoids wasting time, money, and resources, and limits the use of organic solvents.

Keywords: UHPLC; histamine; biogenic amine; food safety; fish products (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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