Biodegradation of Di (2-Ethylhexyl) Phthalate by a novel Enterobacter spp. Strain YC-IL1 Isolated from Polluted Soil, Mila, Algeria

Imane Lamraoui, Adel Eltoukhy, Junhuan Wang, Messaouda Lamraoui, Amer Ahmed, Yang Jia, Tiegang Lu and Yanchun Yan
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Imane Lamraoui: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Adel Eltoukhy: Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Assiut 71524, Egypt
Junhuan Wang: Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
Messaouda Lamraoui: Department of Microbiology, Faculty of Nature Science and Life, University of Bejaia, Bejaia 0600, Algeria
Amer Ahmed: Department of Life Sciences, University of Siena, 53100 Siena, Italy
Yang Jia: Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
Tiegang Lu: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yanchun Yan: Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

IJERPH, 2020, vol. 17, issue 20, 1-15

Abstract: Di-(2-ethylhexyl) phthalate (DEHP) is one of the phthalic acid ester representatives and is mainly used as a plasticizer to endow polyvinyl chloride plastics with desirable physical properties. It is synthesized in massive amounts worldwide. Many studies have proved the adverse effects of DEHP on human health and wildlife. DEHP is labeled as an endocrine disruptor which causes human reproductive problems. Enterobacter spp. YC-IL1, a novel isolated strain from contaminated soil, was identified by 16S rRNA gene analysis and electronic microscope. It is capable of efficiently degrading DEHP (100%) and a wide range of phthalic acid ester PAEs, particularly those containing side chains with branches, or ring structures such as dutylbenzyl phthalate and dicyclohexyl phthalate, which are hard to degrade, with, respectively, 81.15% and 50.69% degradation after 7 days incubation. YC-IL1 is an acido-tolerant strain which remained in pH values lower than pH 5.0 with the optimum pH 7.0 and temperature 30 °C. The DEHP metabolites were detected using HPLC-QQQ and then the degradation pathway was tentatively proposed. Strain YC-IL1 showed high DEHP degradation rate in artificially contaminated soil with 86% removed in 6 days. These results indicate the application potential of YC-IL1 in bioremediation of PAE-polluted sites, even the acidic ones.

Keywords: Di (2-Ethylhexyl) phthalate; biodegradation; Enterobacter spp.; endocrine disruptor; degradation pathway; bioremediation (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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