Batch-Mode Analysis of Thermophilic Methanogenic Microbial Community Changes in the Overacidification Stage in Beverage Waste Treatment

Shuhei Matsuda, Takahiro Yamato, Yoshiyuki Mochizuki, Yoshinori Sekiguchi and Takashi Ohtsuki
Additional contact information
Shuhei Matsuda: Graduate School of Medicine, Engineering and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, Japan
Takahiro Yamato: Graduate School of Medicine, Engineering and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, Japan
Yoshiyuki Mochizuki: Iwata Chemical Co. Ltd., Iwata, Shizuoka 438-0078, Japan
Yoshinori Sekiguchi: Iwata Chemical Co. Ltd., Iwata, Shizuoka 438-0078, Japan
Takashi Ohtsuki: Graduate School of Medicine, Engineering and Agricultural Sciences, University of Yamanashi, Kofu, Yamanashi 400-8510, Japan

IJERPH, 2020, vol. 17, issue 20, 1-13

Abstract: Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus , Defluviitoga tunisiensis , Acetomicrobium mobile , and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification.

Keywords: batch culture; beverage waste; methanogenic microbial community; overacidification (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/17/20/7514/pdf (application/pdf)
https://www.mdpi.com/1660-4601/17/20/7514/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:17:y:2020:i:20:p:7514-:d:428653

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().