Hospital Wastewater—Important Source of Multidrug Resistant Coliform Bacteria with ESBL-Production

Kristína Lépesová, Petra Olejníková, Tomáš Mackuľak, Klára Cverenkárová, Monika Krahulcová and Lucia Bírošová
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Kristína Lépesová: Faculty of Chemical and Food Technology, Department of Nutrition and Food Quality Assessment, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Petra Olejníková: Faculty of Chemical and Food Technology, Institute of Biochemistry and Microbiology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Tomáš Mackuľak: Department of Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Klára Cverenkárová: Faculty of Chemical and Food Technology, Department of Nutrition and Food Quality Assessment, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Monika Krahulcová: Faculty of Chemical and Food Technology, Department of Nutrition and Food Quality Assessment, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Lucia Bírošová: Faculty of Chemical and Food Technology, Department of Nutrition and Food Quality Assessment, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia

IJERPH, 2020, vol. 17, issue 21, 1-14

Abstract: This work compares the prevalence of antibiotic resistant coliform bacteria in hospital wastewater effluents in Slovak (SR) and Czech Republic (?R). It also describes selected antibiotic resistant isolates in view of resistance mechanism and virulence factor. The highest number of multidrug resistant bacteria was detected in samples from the hospital in Valašské Mezi?í?í (?R). More than half of resistant isolates showed multidrug resistance phenotype as well as strong ability to form biofilm. In 42% of isolates efflux pump overproduction was detected together with tetA and tetE genes. The production of extended-spectrum β-lactamases in coliform isolates was encoded mainly by bla TEM , bla CTX-M-2 and bla CTX-M-8/25 genes. About 62% of resistants contained a combination of two or more extended spectrum beta-lactamases (ESBL) genes. Our results strengthen the fact that hospital effluents are a source of multidrug resistant bacteria which can spread their resistance genes to other bacteria in wastewater treatment plants (WWTPs). Accordingly, hospital wastewater should be better treated before it enters urban sewerage.

Keywords: antibiotic resistance; hospital wastewaters; ESBL; biofilm; efflux pumps (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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