First Report of Canine Infection by Leishmania ( Viannia ) guyanensis in the Brazilian Amazon

Francisco J. A. Santos, Luciana C. S. Nascimento, Wellington B. Silva, Luciana P. Oliveira, Walter S. Santos, Délia C. F. Aguiar and Lourdes M. Garcez
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Francisco J. A. Santos: Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, Pará, Brazil
Luciana C. S. Nascimento: Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, Pará, Brazil
Wellington B. Silva: Centro Nacional de Primatas, Instituto Evandro Chagas, Ministério da Saúde, Secretaria de Vigilância em Saúde, Ananindeua 67030-000, Pará, Brazil
Luciana P. Oliveira: Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, Pará, Brazil
Walter S. Santos: Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, Pará, Brazil
Délia C. F. Aguiar: Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, Pará, Brazil
Lourdes M. Garcez: Seção de Parasitologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua 67030-000, Pará, Brazil

IJERPH, 2020, vol. 17, issue 22, 1-9

Abstract: The American cutaneous (CL) and visceral leishmaniasis (VL) are zooanthroponoses transmitted by sand flies. Brazil records thousands of human leishmaniasis cases annually. Dogs are reservoirs of Leishmania infantum , which causes VL, but their role in the transmission cycle of CL is debatable. Wild mammals are considered reservoirs of the aetiological agents of CL ( Leishmania spp.). Objective: To describe the aetiology of leishmaniasis in dogs in an endemic area for CL and VL in the Amazon, Brazil. Methods: Clinical evaluation and blood collection of 40 dogs from the villages Ubim (20) and Socorro (20), city of Tomé-Açu, state of Pará, were carried out. The DNA extracted from the blood was used for PCR with Leishmania -specific primers targeting the hsp70-234 gene sequence. Products were sequenced (ABI3500XL), and the sequences were aligned, edited (BioEdit), and analyzed (Blastn). Results: Of the 34 amplified samples, 21 were sequenced, namely Leishmania infantum (12), L. guyanensis (5), L. braziliensis (3), and Leishmania sp. (01). Conclusion: Given the diversity of circulating pathogens, elucidation of the role of the dog in the Leishmania spp. cycle in Amazonian villages is imperative to the surveillance of CL in the region. We present the first report in Brazil, confirmed by sequencing, of canine infection by L. guyanensis , a species highly resistant to treatment in humans, with the drug of first choice (Glucantime ® ).

Keywords: leishmaniasis; reservoirs; dog; Leishmania spp.; aetiology; epidemiology (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

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