Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues

Luciene Cristina Figueiredo, Bruno Bueno-Silva, Cristiana Fernandes Plutarco Nogueira, Leonardo Carneiro Valadares, Katia Marina Morilla Garcia, Givelton Coimbra da Luz Filho, Luciano Milanello, Felipe Machado Esteves, Jamil Awad Shibli and Tamires Szeremeske Miranda
Additional contact information
Luciene Cristina Figueiredo: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Bruno Bueno-Silva: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Cristiana Fernandes Plutarco Nogueira: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Leonardo Carneiro Valadares: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Katia Marina Morilla Garcia: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Givelton Coimbra da Luz Filho: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Luciano Milanello: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Felipe Machado Esteves: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Jamil Awad Shibli: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil
Tamires Szeremeske Miranda: Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil

IJERPH, 2020, vol. 17, issue 23, 1-9

Abstract: This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups ( p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups ( p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups ( p > 0.05). For all other studied biomarkers, no significant differences were observed between groups ( p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses.

Keywords: periodontitis; peri-implantitis; biomarkers (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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