Analytical Bias in the Measurement of Plasma 25-Hydroxyvitamin D Concentrations in Infants

Kristina Rueter, Lucinda J. Black, Anderson Jones, Max Bulsara, Michael W. Clarke, Cristina Gamez, Ee M. Lim, Debra J. Palmer, Susan L. Prescott and Aris Siafarikas
Additional contact information
Kristina Rueter: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia
Lucinda J. Black: School of Public Health, Curtin University, Perth 6102, Australia
Anderson Jones: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia
Max Bulsara: Institute for Health Research, University of Notre Dame, Fremantle 6160, Australia
Michael W. Clarke: Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth 6009, Australia
Cristina Gamez: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia
Ee M. Lim: Sir Charles Gairdner Hospital, Department of Endocrinology, Perth 6009, Australia
Debra J. Palmer: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia
Susan L. Prescott: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia
Aris Siafarikas: Division of Paediatrics, School of Medicine, The University of Western Australia, Perth 6009, Australia

IJERPH, 2020, vol. 17, issue 2, 1-12

Abstract: Hypovitaminosis D is prevalent worldwide; however, analytical bias in the measurement of circulating 25-hydroxyvitamin D (25(OH)D) concentrations may affect clinical treatment decisions and research. We performed parallel plasma 25(OH)D analyses using the Abbott Architect i2000 chemiluminescent immunoassay (CIA) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) for paired samples from the same infants at 3 ( n = 69), 6 ( n = 79) and 12 months ( n = 73) of age. To test agreement, we used Lin’s concordance correlation coefficient and corresponding 95% confidence interval, Bland–Altman’s limits of agreement, and Bradley–Blackwood (BB) test. Agreement was high at 3 months (coefficient between difference and mean −0.076; BB F = 0.825; p = 0.440), good at 12 months (−0.25; BB F = 2.41; p = 0.097) but missing at 6 months of age (−0.39; BB F = 12.30; p < 0.001). Overall, 18 infants had disparate results based on the cut-off point for vitamin D deficiency (25(OH)D < 50 nmol/L), particularly at three months, with seven (10%) infants deficient according to CIA but not LC–MS/MS, and four (6%) deficient by LC–MS/MS but not CIA. To our knowledge, this is the first study to show that the reported 25(OH)D concentration may be influenced by both age and assay type. Physicians and researchers should be aware of these pitfalls when measuring circulating 25(OH)D concentrations in infants and when developing treatment plans based on measured vitamin D status.

Keywords: 25-hydroxyvitamin D; analytical bias; infants; vitamin D (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/17/2/412/pdf (application/pdf)
https://www.mdpi.com/1660-4601/17/2/412/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:17:y:2020:i:2:p:412-:d:306378

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().