Evaluation of Microbial Adhesion and Biofilm Formation on Nano-Structured and Nano-Coated Ortho-Prosthetic Materials by a Dynamic Model

Simone Leonetti, Benedetta Tuvo, Beatrice Campanella, Stefano Legnaioli, Massimo Onor, Emilia Bramanti, Michele Totaro, Angelo Baggiani, Serena Giorgi, Gaetano Pierpaolo Privitera, Nicola Piolanti, Paolo Domenico Parchi and Beatrice Casini
Additional contact information
Simone Leonetti: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Benedetta Tuvo: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Beatrice Campanella: Institute of Chemistry of Organometallic Compounds, National Research Council, via Giuseppe Moruzzi, 1-56124 Pisa, Italy
Stefano Legnaioli: Institute of Chemistry of Organometallic Compounds, National Research Council, via Giuseppe Moruzzi, 1-56124 Pisa, Italy
Massimo Onor: Institute of Chemistry of Organometallic Compounds, National Research Council, via Giuseppe Moruzzi, 1-56124 Pisa, Italy
Emilia Bramanti: Institute of Chemistry of Organometallic Compounds, National Research Council, via Giuseppe Moruzzi, 1-56124 Pisa, Italy
Michele Totaro: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Angelo Baggiani: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Serena Giorgi: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Gaetano Pierpaolo Privitera: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Nicola Piolanti: Orthopaedic and Traumatology Division, Azienda ospedaliera-Universitaria Pisana, via Roma, 67-56126 Pisa, Italy
Paolo Domenico Parchi: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy
Beatrice Casini: Department of Translational Research, N.T.M.S., University of Pisa, via San Zeno, 37/39-56127 Pisa, Italy

IJERPH, 2020, vol. 17, issue 3, 1-11

Abstract: The bio-engineering technologies of medical devices through nano-structuring and coating was recently proposed to improve biocompatibility and to reduce microbial adhesion in the prevention of implantable device-related infections. Our aim was to evaluate the ability of new nano-structured and coated materials to prevent the adhesion and biofilm formation, according to the American Standard Test Method ASTM-E2647-13. The materials composition was determined by X-ray Fluorescence and Laser Induced Breakdown Spectroscopy. Silver release was evaluated by Inductively Coupled Plasma Mass Spectrometry analysis. The gene expression levels of the Quorum Sensing Las and Rhl system were evaluated by the ΔΔCt method. The Log bacterial density (Log CFU/cm 2 ) on TiAl6V4 was 4.41 ± 0.76 and 4.63 ± 1.01 on TiAl6V4-AgNPs compared to 2.57 ± 0.70 on CoCr and 2.73 ± 0.61 on CoCr-AgNPs (P < 0.0001, A.N.O.V.A.- one way test). The silver release was found to be equal to 17.8 ± 0.2 µg/L after the batch phase and 1.3 ± 0.1 µg/L during continuous flow. The rhlR gene resulted in a 2.70-fold increased expression in biofilm growth on the silver nanoparticles (AgNPs) coating. In conclusion, CoCr showed a greater ability to reduce microbial adhesion, independently of the AgNPs coating. The silver release resulted in promoting the up-regulation of the Rhl system. Further investigation should be conducted to optimize the effectiveness of the coating.

Keywords: biofilm; Drip Flow Biofilm Reactor; implantable device-related infections; titanium and cobalt-chrome nano-structured materials; silver nanoparticles; Quorum Sensing (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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