Apoptotic Effect of 1800 MHz Electromagnetic Radiation on NIH/3T3 Cells

Dan-Yang Li, Jing-Dong Song, Zhao-Yuan Liang, Kiana Oskouei, Xiang-Qian Xiao, Wen-Zhe Hou, Jin-Tao Li, Yi-Shu Yang, Ming-Lian Wang and Manuel Murbach
Additional contact information
Dan-Yang Li: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Jing-Dong Song: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Zhao-Yuan Liang: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Kiana Oskouei: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Xiang-Qian Xiao: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Wen-Zhe Hou: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
Jin-Tao Li: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Yi-Shu Yang: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Ming-Lian Wang: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China
Manuel Murbach: IT’IS Foundation, Zeughausstrasse 43, 8004 Zurich, Switzerland

IJERPH, 2020, vol. 17, issue 3, 1-11

Abstract: To investigate the effect of 1800 MHz electromagnetic radiation (EMR) on apoptosis, we exposed NIH/3T3 cells at 1800 MHz with a specific absorption rate (SAR) of 2 W/kg intermittently for 12, 24, 36, and 48 h. After exposure, Cell Counting Kit-8 (CCK-8) and flow cytometry were used to detect cell viability and apoptosis; the expression of p53, a molecule with the key role in apoptosis, was measured by real-time qPCR, western blot, and immunofluorescence; and images of the structure of the mitochondria, directly reflecting apoptosis, were captured by electron microscopy. The results showed that the viability of cells in the 12, 36, and 48 h exposure groups significantly decreased compared with the sham groups; after 48 h of exposure, the percentage of late apoptotic cells in the exposure group was significantly higher. Real-time qPCR results showed that p53 mRNA in the 48 h exposure group was 1.4-fold of that in the sham group; significant differences of p53 protein fluorescence expression were observed between the exposure groups and the sham groups after 24 h and 48 h. The mitochondrial swelling and vesicular morphology were found in the electron microscopy images after 48 h exposure. These findings demonstrated 1800 MHz, SAR 2 W/kg EMR for 48 h may cause apoptosis in NIH/3T3 cells and that this apoptosis might be attributed to mitochondrial damage and upregulation of p53 expression.

Keywords: electromagnetic radiation; cell apoptosis; mitochondria; p53 (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
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