Variation in the Occurrence of fimA Genotypes of Porphyromonas gingivalis in Periodontal Health and Disease

Manohar Kugaji, Uday Muddapur, Kishore Bhat, Vinayak Joshi, Manjunath Manubolu, Kavitha Pathakoti, Malleswara Rao Peram and Vijay Kumbar
Additional contact information
Manohar Kugaji: Central Research Laboratory, Maratha Mandal’s NGH Institute of Dental Sciences & Research Centre, Bauxite Road, Belagavi, Karnataka 590010, India
Uday Muddapur: B.V. Bhoomaraddi College of Engineering and Technology, KLE Technological University, Vidya Nagar, Hubballi, Karnataka 580031, India
Kishore Bhat: Central Research Laboratory, Maratha Mandal’s NGH Institute of Dental Sciences & Research Centre, Bauxite Road, Belagavi, Karnataka 590010, India
Vinayak Joshi: Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH 4312, USA
Manjunath Manubolu: Aquatic Ecology Laboratory, Department of Evolution, Ecology and Organismal Biology, The Ohio State University, Columbus, OH 4312, USA
Kavitha Pathakoti: Interdisciplinary Center for Nanotoxicity, Department of Chemistry, Physics and Atmospheric Sciences, Jackson State University, Jackson, MS 39217, USA
Malleswara Rao Peram: Central Research Laboratory, Maratha Mandal’s NGH Institute of Dental Sciences & Research Centre, Bauxite Road, Belagavi, Karnataka 590010, India
Vijay Kumbar: Central Research Laboratory, Maratha Mandal’s NGH Institute of Dental Sciences & Research Centre, Bauxite Road, Belagavi, Karnataka 590010, India

IJERPH, 2020, vol. 17, issue 6, 1-8

Abstract: Porphyromonas gingivalis is regarded as a “keystone pathogen” in periodontitis. The fimbria assists in the initial attachment, biofilm organization, and bacterial adhesion leading to the invasion and colonization of host epithelial cells. The present study aimed to investigate the occurrence of fimA genotypes in patients with chronic periodontitis and healthy individuals in the Indian population, and to study their association with the number of P. gingivalis cells obtained in subgingival plaque samples of these subjects. The study comprised 95 samples from the chronic periodontitis (CP) group and 35 samples from the healthy (H) group, which were detected positive for P. gingivalis in our previous study. Fimbrial genotyping was done by PCR and PCR-restriction fragment length polymorphism (RFLP). The fimA type II was more prevalent in the CP group (55.89%), followed by type IV (30.52%), whereas in the H group, type I was the most prevalent fimbria (51.42%). The quantity of P. gingivalis cells increased with the presence of fimA types II and III. Our results suggest a strong relationship between fimA types II and IV and periodontitis, and between type I and the healthy condition. The colonization of organisms was increased with the occurrence of type II in deep periodontal sites, which could play an important role in the progression of the disease.

Keywords: fimbriae; polymerase chain reaction (PCR); periodontitis; Porphyromonas gingivalis; virulence (search for similar items in EconPapers)
JEL-codes: I I1 I3 Q Q5 (search for similar items in EconPapers)
Date: 2020
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.mdpi.com/1660-4601/17/6/1826/pdf (application/pdf)
https://www.mdpi.com/1660-4601/17/6/1826/ (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:gam:jijerp:v:17:y:2020:i:6:p:1826-:d:331330

Access Statistics for this article

IJERPH is currently edited by Ms. Jenna Liu

More articles in IJERPH from MDPI
Bibliographic data for series maintained by MDPI Indexing Manager ().